At1g78840 Antibody
Description
Research Applications
The antibody is validated for detecting At1g78840 in Arabidopsis tissues via:
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ELISA: Quantifies antigen levels in solution-phase assays.
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WB: Identifies At1g78840 in lysates or immunoprecipitates, ensuring specificity through antigen recognition.
Key Considerations
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Cross-reactivity: Limited data on cross-reactivity with non-Arabidopsis species.
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Optimal Dilution: Adjustments may be required based on experimental conditions (e.g., sample type, detection method).
Limitations and Challenges
While the antibody is tailored for Arabidopsis research, broader antibody specificity challenges observed in related studies highlight potential caveats:
General Antibody Specificity Concerns
Commercial antibodies targeting receptors like the angiotensin II type 1 receptor (AT1R) often exhibit non-specific binding in mouse tissues . Key issues include:
Note: At1g78840 Antibody specificity has not been explicitly tested in non-Arabidopsis models.
Broader Context in Antibody Research
Recent advancements in antibody engineering and validation include:
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Structure-Function Relationships: The constant region (Fc) influences antigen binding and signaling, enabling diverse mechanisms of action .
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CDR3 Diversity: The heavy-chain complementarity-determining region 3 (HCDR3) is critical for target specificity, though identical sequences can arise from distinct V-D-J rearrangements .
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Engineered Antibodies: pH-dependent binding (e.g., sweeping antibodies) enhances therapeutic targeting by mimicking receptor-ligand interactions .
