At5g62970 Antibody

Absence of Direct References to "At5g62970 Antibody"

No search results or publications explicitly mention an antibody targeting "At5g62970," a designation that resembles a gene identifier (e.g., Arabidopsis thaliana genes follow the format "AtXgXXXXX"). This suggests one of the following scenarios:

• Mislabeling or Typographical Error: The compound name may be misspelled or conflated with similar identifiers (e.g., clone names like "At5" in ).

• Niche or Emerging Research: The antibody might be part of unpublished or highly specialized studies not indexed in the reviewed sources.

Monoclonal Antibody At5

Search result describes monoclonal antibody At5, initially developed against chordin in sturgeon fish, which cross-reacts with human neural antigens:

• Targets:

  • dMAG (a derivative of myelin-associated glycoprotein) in human brain tissue.

  • Proteoglycans phosphacan and neurocan in oligodendroglioma tumors .

• Applications: Used in histopathology to differentiate oligodendroglioma from astrocytoma via immunostaining .

Antibody Clone 5E4

Result highlights anti-glucocorticoid receptor (GR) antibody clone 5E4, which exhibits cross-reactivity with AMPD2 and TRIM28 proteins, underscoring challenges in antibody specificity validation.

Specificity and Validation Challenges

• Antibodies like clone 5E4 ( ) and commercial anti-DEFA5 clones ( ) demonstrate the critical need for rigorous validation (e.g., knockout cell lines, mass spectrometry) to confirm target specificity.

• Up to 75% of commercial antibodies may fail in specific applications, as shown in large-scale studies like YCharOS ( ).

Recommendations for Further Investigation

1. Verify Compound Nomenclature: Confirm whether "At5g62970" refers to a gene, epitope, or antibody clone.

2. Explore Orthogonal Databases:

   • Consult specialized repositories like the Developmental Studies Hybridoma Bank (DSHB) or the Antibody Registry.

   • Screen patent databases (e.g., USPTO, WIPO) for proprietary antibodies.

3. Experimental Validation: If the antibody exists in unpublished contexts, perform:

   • Western blotting with positive/negative controls.

   • Immunoprecipitation-mass spectrometry to identify off-target binding ( ).