At3g26920 Antibody

Target Identification

The At3g26920 gene in Arabidopsis thaliana encodes a protein with the UniProt identifier Q9LJF9. While its precise biological function remains uncharacterized in public databases, antibodies against this target enable researchers to investigate its role in plant development, stress responses, or metabolic pathways .

Development and Validation

• Production: Generated by immunizing rabbits with recombinant At3g26920 protein, followed by affinity purification to enhance specificity .

• Specificity: Recognizes the target protein in ELISA and WB, as confirmed by antigen-binding assays .

• Advantages: Polyclonal structure allows detection of multiple epitopes, increasing sensitivity for denatured proteins in WB .

Protein Expression Analysis

• Western Blot: Used to detect At3g26920 protein levels in Arabidopsis tissue lysates, aiding in studies of gene regulation under varying experimental conditions .

• ELISA: Quantifies protein abundance in crude extracts, enabling high-throughput screening .

Functional Studies

While direct studies using this antibody are not yet published, analogous tools in plant research have been critical for:

• Mapping protein interactions via immunoprecipitation .

• Investigating subcellular localization through immunofluorescence .

Quality Assurance

• Validation: Specificity confirmed through antigen-binding tests; no cross-reactivity data available for unrelated plant proteins .

• Stability: Maintains activity for >1 year when stored at -80°C in glycerol-containing buffer .

Limitations and Considerations

• Species Restriction: Reactivity limited to Arabidopsis thaliana; not validated for other plant species .

• Application Scope: Untested in advanced techniques like immunohistochemistry or flow cytometry.