FEI2 Antibody

Clarifying FEI2 vs. FEZ2

• FEI2: A receptor-like kinase in Arabidopsis thaliana critical for seed mucilage formation, cell wall integrity, and cellulose biosynthesis. It interacts with SOS5 (a GPI-anchored arabinogalactan protein) to regulate cellulosic ray formation in seed coats .

• FEZ2: A human protein involved in neuronal development, fasciculation, and elongation. Antibodies against FEZ2 are commercially available (e.g., Sigma-Aldrich ABN341, ABN342), but no FEI2-specific antibodies are mentioned in the provided sources .

Key Issue: The search results contain no data on FEI2 antibodies. Instead, they describe FEZ2 antibodies and FEI2’s role in plant biology.

FEI2 Function and Research Findings

FEI2 is a transmembrane protein with a leucine-rich repeat (LRR) domain and cytosolic kinase activity. Its role in seed mucilage development includes:

• Cellulosic Ray Formation: FEI2 localizes SOS5 to the plasma membrane, enabling interactions with pectins and cellulose synthesis via CESA5 .

• Mucilage Defects: fei2 mutants show reduced crystalline cellulose in adherent mucilage layers, leading to mal-partitioned pectins and impaired mucilage adherence .

• Non-Redundant Role: Unlike FEI1, FEI2 is indispensable for seed mucilage structure, as fei2 single mutants phenocopy fei1 fei2 double mutants .

FEZ2 Antibodies: Available Products

While FEI2-specific antibodies are absent in the sources, FEZ2 antibodies are listed in Sigma-Aldrich’s catalog:

Note: These antibodies target FEZ2, not FEI2, and are unrelated to plant biology .

Critical Observations

• Terminology Conflict: The query conflates FEI2 (plant) and FEZ2 (human). No FEI2 antibodies are documented in the provided sources.

• Research Gaps: FEI2 remains understudied in terms of antibody development, likely due to its plant-specific role.

• Future Directions: Development of FEI2-specific antibodies would require recombinant protein production or immunization strategies targeting its LRR or kinase domains.