FGF10 Antibody
Description
What is FGF10 Antibody?
FGF10 antibodies are immunoglobulin-based reagents designed to detect and quantify FGF10 in experimental settings. These antibodies bind specifically to FGF10, enabling its visualization in assays such as Western blot (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). FGF10 is encoded by the FGF10 gene (UniProt ID: O15520) and shares 92–95% sequence homology across human, mouse, and rat species .
Research Applications
FGF10 antibodies are widely used in:
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Developmental Biology: Studying limb bud formation, lung branching morphogenesis, and salivary gland development .
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Cancer Research: Investigating FGF10/FGFR2 signaling in cholangiocarcinoma (CCA) metastasis and therapeutic targeting .
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Inflammation Studies: Analyzing cross-talk between FGF10 and immune responses in lung injury and fibrosis .
Key Findings Using FGF10 Antibodies
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In CCA: FGF10 promotes metastasis via Akt/mTOR and VEGF/Slug pathways. FGFR inhibitors (e.g., infigratinib) suppress FGF10-driven cell migration .
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Lung Development: Fgf10 heterozygosity alters immune gene expression in alveolar type 2 (AT2) cells, exacerbating hyperoxia-induced injury .
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Wound Healing: FGF10 mitigates inflammation by blocking HMGB1 translocation in bronchial epithelial cells .
Validation and Quality Control
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Western Blot: Detects FGF10 at ~20–40 kDa in lung carcinoma (A549) and HEK-293 cells .
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IHC: Localizes FGF10 in human prostate tissue and mouse embryonic spinal cord .
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ELISA: Paired antibodies (e.g., AF345 + MAB3451) achieve high sensitivity in FGF10 quantification .
Challenges and Considerations
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Cross-Reactivity: Some antibodies cross-react with FGF7 due to structural homology .
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Storage: Sodium azide (0.02–0.1%) is recommended for preservation but requires careful handling .
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Species Specificity: Mouse-specific antibodies (e.g., AF6224) show <100% cross-reactivity with rat FGF10 .
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- A recent study reported the ophthalmological and genetic analysis of a 19-year-old woman and her relatives affected by a syndrome. A novel probably pathogenic variant was identified in the FGF10 gene. PMID: 29053399
- Research has demonstrated a significant association between polymorphisms in the FGFR2 and MAP3K1 genes and breast cancer. PMID: 29372690
- Data suggests that the strongest signal of association at 5p12 is mediated through coordinated activation of FGF10 and MRPS30, two potential candidate genes involved in breast cancer pathogenesis. PMID: 27640304
- Fgf10 signaling plays a critical role in the formation of lipofibroblasts during the later stages of lung development. PMID: 26511927
- Expression of Fibroblast Growth Factor 10 is correlated with poor prognosis in gastric adenocarcinoma. PMID: 26268776
- FGF10 has a protective effect on neurons against oxygen-glucose deprivation injury by inducing heme oxygenase-1. PMID: 25446127
- Data indicates that autocrine activation of FGF signaling is an essential mechanism in promoting Pten-deficient skin tumors. PMID: 24582960
- The therapeutic potential of FGF10 treatment is under investigation. PMID: 24865969
- FGF10 plays a significant role in tumor growth through both paracrine and autocrine mechanisms. PMID: 24320134
- Research suggests that immunohistochemistry using FGF10, FGFR2b, or SHH could be valuable in differentiating CCAM from type I PPB in children presenting with focal cystic lung lesions. PMID: 24004862
- Paracrine FGF10 signaling stimulates the differentiation of human stem cells into urothelial cells. PMID: 23949743
- High FGF10 expression is associated with ameloblastoma. PMID: 24002438
- Three FGF10 single nucleotide polymorphisms in complete linkage disequilibrium--rs339501, rs12517396, and rs10462070--were associated with extreme myopia in the Japanese population. PMID: 24265547
- FGF-10 expression during the development of the human hindgut and anorectum suggests a potential role in hindgut and anorectal morphogenesis. PMID: 23774963
- The sclera of myopic eyes had higher FGF10 levels. The risk G allele of SNP rs339501 was associated with extreme myopia in humans and caused a higher gene expression in the luciferase assay. PMID: 23599340
- Studies have investigated the posttranslational and transcriptional mechanisms underlying the stimulation of P-glycoprotein function and expression by keratinocyte growth factor-2 (KGF2), potentially contributing to the beneficial effects of KGF2 in intestinal inflammatory disorders. PMID: 23328208
- Individuals with tooth agenesis had an increased risk of a family history of cancer. Tooth agenesis was associated with a positive self-reported family history of cancer and variants in FGF10. PMID: 23169889
- The identification of FGF-10 at both protein and mRNA levels in ovaries from fetuses, girls, and women suggests a role for FGF-10 in preantral follicle development. FGF-10 is expressed in both granulosa cells and oocytes. PMID: 22877940
- Research suggests that common variants in FGF10 increase the risk for a wide range of non-syndromic limb deficiencies. PMID: 22965740
- [review] FGF10 mediates biological responses by activating FGF receptor 2b (FGFR2b) in a paracrine manner. PMID: 21696361
- The relationship between human ISL1 and FGF10 within the embryonic time window during which the linear heart tube remodels into four chambers, was examined. PMID: 22303449
- A study investigated the pulmonary functions of COPD patients heterozygous for loss of function mutations in the FGF10 gene. These patients showed a significant decrease in lung function parameters compared to control values. PMID: 21742743
- Gremlin-mediated BMP inhibition results in activation of epithelial cells and transient fibrosis, but also induction of epithelium-protective FGF10. PMID: 20705941
- FGF2 and FGF10 regulate the migratory activity of ovine trophoblast cells through MAPK-dependent pathways. PMID: 21310815
- FGF10 can promote the adipogenesis effect in situ. PMID: 19915940
- There was no association among gene FGFR1 rs13317, p. E467K, p. M369I, p. S393S and gene FGF10 rs1448037 and nonsyndromic cleft lip with or without palate in the Chinese population. PMID: 19727229
- These results suggest that Thr-114 is a crucial functional residue for FGF10, and mutating Thr-114 to Ala or Arg would significantly decrease the receptor-binding affinity and biological activity of FGF10. PMID: 20036575
- FGF10 upregulates Na(+)-K(+)-exchanging ATPase via the MAPK pathway. PMID: 12804770
- FGF-10 attenuates H2O2-induced alveolar epithelium DNA damage through mechanisms involving activation of the Grb2-SOS/Ras/RAF-1/ERK1/2 pathway and DNA repair. PMID: 14975937
- Fgf10 mRNA is overexpressed in a subset of human breast carcinomas. PMID: 15208658
- Data validates the symmetric two-end model of fibroblast growth factor (FGF) receptor (FGFR) dimerization and FGF binding, and argues against the asymmetric model of FGFR dimerization. PMID: 15632068
- Researchers suggest that haploinsufficiency for FGF10 during a critical stage of development results in ALSG. PMID: 15654336
- Human embryonic pancreatic mesenchyme expresses FGF10, which could be used to expand human embryonic pancreatic epithelial cells. PMID: 15690149
- Human recombinant FGF10 maintains murine Notch activation and induces the expansion of murine pancreatic precursors while blocking their differentiation. PMID: 16323074
- The localization of the FGF-10 receptor to the urothelial layer is clinically significant because intravesical administration of FGF-10 may provide a means to control the turnover of transitional epithelium in bladder disorders such as interstitial cystitis. PMID: 16597614
- Activation of Toll-like receptor 2 (TLR2) or Toll-like receptor 4 (TLR4) inhibited FGF-10 expression, leading to abnormal saccular airway morphogenesis. PMID: 17071719
- R80S and G138E mutations are associated with aplasia of the lacrimal and salivary glands. PMID: 17213838
- A nuclear localization signal-like motif of FGF-10 is a partial determinant of its intracellular distribution and is necessary for its mitogenic activity. PMID: 17471512
- Trophoblast outgrowth and invasion (part of placental villi sprouting) at the fetal maternal interface is in part under delicate control of FGF 10 and Sprouty 2. PMID: 17496316
- Results suggest that Lacrimo-auriculo-dento-digital syndrome is caused by reduced activity of the fibroblast growth factor 10 (FGF10)-FGF receptor 2 signaling pathway. PMID: 17682060
- Expression in diverse niches of the adult brain of Fgf10-lacZ reporter mice implicates Fgf10 in the control of neurogenesis and/or conservation of neurogenic potential. PMID: 18329286
- It cannot be ruled out that other genes involved in the signaling pathway of FGF10 may contribute to the formation of these congenital malformations. PMID: 18587586
- Stromal FGF10 induces migration and invasion in pancreatic cancer cells through interaction with FGFR2, leading to a poor prognosis. PMID: 18594526
- A family-based approach revealed an intronic variation of the FGF10 gene causing aplasia of lacrimal and salivary glands-syndrome. PMID: 19102732
- Disruption of the localized pattern of mesenchymal fibroblast growth factor 10 expression results in impairment of lung branching morphogenesis, which progresses to emphysematous airspaces in adults. PMID: 19115389
- Fgf10 is a strong causative candidate for defects observed in Apert syndrome since its genetic knockdown in a mouse model of Apert syndrome results in the rescue of the skeletal and visceral defects associated with this congenital disease. PMID: 18773495
- An analysis of the distribution and fate of Fgf10-expressing cells in the adult mouse brain suggests that Fgf10 may have critical regulatory roles in stem cell function and generation of new neurons in diverse areas of the adult brain. PMID: 18773495
- TGFbeta1 caused a rapid and transient decrease in Fgf10 mRNA levels in primary prostatic cells. Deletion analysis of the Fgf10 promoter identified a region that mediated a proportion of promoter activity as well as promoter down-regulation by TGFbeta1. PMID: 14726452
Q&A
How to Select the Optimal FGF10 Antibody for Epithelial-Mesenchymal Interaction Studies?
When investigating FGF10's role in epithelial-mesenchymal crosstalk, prioritize antibodies validated for species-specific reactivity and application compatibility. For mouse embryonic studies, the AF6224 antibody detects FGF10 in frozen sections of E13 embryos with localization to spinal cord tissues . In human cancer models, MAB3451 demonstrates specificity for recombinant human FGF10 (Cys37-Ser208) and pairs with AF345 for ELISA development . Cross-reactiveness assessments should include:
Methodological Tip: Pre-test antibodies in knockout controls (e.g., Fgf10−/− mice) to confirm absence of non-specific binding .
Validating Antibody Specificity in Western Blot: Why Do Multiple Bands Appear?
Non-specific bands in WB often arise from post-translational modifications or antibody cross-reactivity. The AF6224 antibody detects a single 20 kDa band in A549 lung carcinoma lysates under reducing conditions , while DF6333 identifies a 23 kDa isoform in human tissues due to glycosylation variance . To resolve discrepancies:
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Use deglycosylation enzymes (e.g., PNGase F) to distinguish glycosylated vs. core protein forms .
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Compare migration patterns across buffer systems (e.g., Immunoblot Buffer Group 8 reduces aberrant migrations) .
Advanced Application: Quantifying FGF10 in Tumor Microenvironments
In pancreatic ductal adenocarcinoma (PDAC), FGF10 serum levels correlate with tumor progression. A multiplex approach combines:
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ELISA: MAB3451/AF345 pair detects FGF10 at sensitivities of 15.6–1,000 pg/mL .
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Spatial IHC: Localize FGF10-producing fibroblasts using AF6224 (5 µg/mL, overnight incubation at 4°C) with tyramide signal amplification .
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Single-cell RNA-seq: Identify FGF10-expressing stromal cells (e.g., macrophages) in PDAC biopsies .
Addressing Contradictory Data: FGF10 in Breast Cancer Subtypes
Conflicting reports on FGF10’s role in ER+ vs. basal-like breast cancers necessitate careful experimental design:
Advanced Tip: Use proximity ligation assays (PLA) to visualize FGF10-FGFR2b interactions in situ .
Methodological Pitfalls: Cross-Reactivity with FGF7 and FGF22
FGF10 shares 53% homology with FGF7, risking antibody cross-reactivity. Mitigation strategies include:
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Peptide Blocking: Pre-incubate DF6333 with FGF7/FGF22 peptides (10-fold molar excess) .
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Kinase Activity Assays: Verify functional specificity by measuring ERK1/2 phosphorylation exclusively in FGFR2b-transfected cells .
Standardizing IHC Protocols for Developmental Studies
For embryonic lung branching morphogenesis:
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Fixation: Immersion-fix E13 mouse embryos in 4% PFA for 24 hr .
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Staining: AF6224 (5 µg/mL) + Anti-Sheep HRP-DAB Kit, with hematoxylin counterstain .
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Validation: Compare signal distribution in Fgf10+/+ vs. Fgf10−/− littermates .
Mechanistic Studies: Linking FGF10 Signaling to Downstream Pathways
To dissect FGF10-FGFR2b signaling:
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Co-Immunoprecipitation (Co-IP): Use MAB3451 to pull down FGF10-FGFR2b complexes from MDA-MB-231 lysates .
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Phosphoproteomics: Map ERK/MAPK activation using phospho-specific antibodies post-FGF10 stimulation (10 ng/mL, 15 min) .
Resolving Discrepancies in Molecular Weight Observations
Reported FGF10 molecular weights vary:
| Source | Observed MW (kDa) | Conditions |
|---|---|---|
| A549 cells (AF6224) | 20 | Reducing, Immunoblot Buffer 8 |
| Human serum (DF6333) | 23 | Non-reducing, RIPA buffer |
Solution: Include recombinant FGF10 (20 kDa, R&D Systems #345-FG) as a migration control .
Multiplexed Detection in Complex Biological Systems
In gastric cancer microenvironments:
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CyTOF: Stain FFPE sections with AF6224 (Metal-conjugated, 1:100) and panel of 35 metal-tagged antibodies .
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Spectral Flow Cytometry: Combine DF6333 (APC) with PD-L1 (FITC) to correlate FGF10+ fibroblasts with immune evasion .
Ethical Considerations in Preclinical Validation
When using xenograft models:
