FGF4 Antibody, Biotin conjugated
Description
Composition and Characteristics of FGF4 Biotin-Conjugated Antibodies
FGF4 biotin-conjugated antibodies are typically polyclonal rabbit antibodies targeting specific epitopes of the FGF4 protein. Key features include:
These antibodies are often purified via affinity chromatography and stored in glycerol-containing buffers with sodium azide as a preservative .
Oncogenic Role in Kaposi’s Sarcoma (KS)
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Amplification Detection: Biotin-conjugated FGF4 antibodies were used in fluorescence in situ hybridization (FISH) to identify FGF4 gene amplification at chromosomal region 11q13 in KS tumors. Co-amplification with INT2 (another oncogene) was observed in 63% of cases .
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Immunohistochemistry: In KS samples, FGF4 protein was detected in neoplastic cells using biotin-streptavidin immunoperoxidase techniques. Weak nonspecific staining in non-neoplastic cells highlighted the importance of controlled validation .
Stem Cell Proliferation Studies
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Mouse Embryonic Stem Cells (mESCs):
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Human Periodontal Ligament Stem Cells (hPDLSCs):
Comparative Performance in Assays
Limitations and Considerations
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Cross-Reactivity: Some antibodies show weak cross-reactivity with non-neoplastic cells, necessitating stringent controls .
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Storage Stability: Sodium azide preservative requires careful handling, and repeated freeze-thaw cycles degrade antibody efficacy .
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Species Specificity: While reactive with human, mouse, and rat samples, performance varies by tissue type and fixation method .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Our research demonstrated that FGFR4 rs2011077 and rs1966265 are associated with the progression from cervical normal tissues to precancerous lesions in Taiwanese women. Furthermore, rs351855 (Gly388Arg) is the only FGFR4 genetic polymorphism linked to patient survival. PMID: 28378614
- Our findings suggest that the oncoprotein HBXIP upregulates FGF4 through activation of the transcription factor Sp1, promoting the migration of breast cancer cells. From a therapeutic perspective, HBXIP could serve as a potential target for breast cancer treatment. PMID: 26828265
- Fibroblasts induce FGF4 expression in ovarian cancer stem-like cells/cancer-initiating cells, enhancing their tumor initiation capacity. PMID: 25329002
- Data indicates that the interaction between Artd1 and Sox2 is critical for the initial stages of the reprogramming process, and early Fgf4 expression is essential for successful generation of induced pluripotent stem cells (iPSCs). PMID: 23939864
- Myoblasts overexpressing FGF-4 exhibit significant changes in cell cycle and pro-angiogenic potential with only minor differences in the expression of myogenic genes. PMID: 21673370
- In vivo stimulation of BT-474 cell growth by progesterone is associated with upregulation of FGF4, which may promote tumor growth and maintenance. PMID: 22237711
- Knockdown of FGFR4 expression resulted in decreased proliferation and increased apoptosis rates in the MKN45 and SGC7901 GC cell lines. PMID: 21567388
- Activation of the human HST-1 gene in transgenic mice induces spermatogenesis and prevents adriamycin-induced testicular toxicity. PMID: 11840335
- FGF4, EGF, and TGFB1 exhibit differential effects on the functional development of stromal layers (progenitor cell-outputs) in acute myeloid leukemia. PMID: 12163055
- FGF4 is upregulated by the OCT3 transcription factor in breast cancer cells. PMID: 12841847
- HST-1 protects male germ cells from apoptosis under heat-stress conditions in a mouse model. PMID: 14980503
- Both myeov and hst (fgf4) are typically located approximately 475-kb apart at band 11q13, a region frequently amplified and overexpressed in various tumors. PMID: 17390055
- FGF-4 accelerates the proliferation rate of mesenchymal stem cells (MSCs) without significantly affecting their pluripotency. PMID: 17852409
- These findings suggest a growth-promoting role for FGF4 in human embryonic stem cells and a possible feedback inhibition mechanism by a novel FGF4 splice isoform that may promote differentiation in later developmental stages. PMID: 18192227
- Implantation of human FGF4-soaked beads is sufficient to restore expression of G1- and S-phase cell-cycle genes and S-phase progression in zebrafish sonic hedgehog (Shh) mutant fin buds. PMID: 18811955
- The combined action of retinoic acid and FGF4 results in the induction of PDX1+ foregut endoderm. PMID: 19277121
