FHL3 Antibody
Description
Introduction
The FHL3 Antibody is a research reagent designed to detect and analyze the Four and a Half LIM Domains 3 (FHL3) protein, a member of the FHL family involved in cellular signaling, myogenesis, and cancer progression. This antibody is widely used in immunological assays to study FHL3’s role in various biological contexts, including tumor development and muscle differentiation.
Cancer Studies
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Gastric Cancer: FHL3 overexpression correlates with poor prognosis and chemotherapy resistance. The antibody was used in immunohistochemistry (IHC) and Western blot (WB) to demonstrate FHL3’s role in epithelial-to-mesenchymal transition (EMT) and multidrug resistance gene 1 (MDR1) upregulation .
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Glioma: FHL3 knockdown experiments employing this antibody revealed its role in glioma cell growth and apoptosis modulation via transcriptional regulation of SOX4 .
Muscle Biology
The antibody has been used to study FHL3’s localization in skeletal muscle. For example, it showed FHL3’s nuclear and focal adhesion localization during myogenesis, interacting with transcription factors like MyoD and CREB .
Key Research Findings
Challenges and Future Directions
Product Specs
Target Background
- This study has established a link between Ang and FHL3 proteins, uncovering a new pathway for regulating astrocytoma progression. PMID: 25659096
- These findings indicate that functional interactions between FHL3 and MT-1X might offer insights into the mechanisms of FHL3-mediated cell proliferation. PMID: 24690879
- PCBP2 can bind to FHL3 mRNA 3'UTR-A and suppress FHL3 expression in T98G glioms cells. PMID: 24998228
- FHL3 enhances Tax1-mediated activation of the Human T-cell leukemia virus type 1 long terminal repeat (LTR) without affecting basal activity and regulates NF-kappaB activation by Tax1 in a cell-specific manner. PMID: 23616667
- Findings suggest that the interaction between Ang and FHL3 could provide insights into the mechanisms of Ang-regulated cell growth and apoptosis. PMID: 22633874
- FHL3 inhibited anchorage-dependent and -independent growth of human breast cancer cells. PMID: 22362714
- FHL3 does not bind to HIF-1alpha or p300, indicating that it regulates transactivation through a novel molecular mechanism. PMID: 22219185
- The second zinc finger motif in LIM4 was responsible for the auto-activation of FHL3. PMID: 20586194
- Data demonstrate that FHL3 (human four-and-a-half LIM-only protein 3) interacts with human phosphatase CDC25B in the cell nucleus. PMID: 12681290
- MZF-1 and FHL3 formed a complex of high molecular mass with additional proteins in the nucleus. Transcriptional repression of FcepsilonRI by MZF-1 required FHL3. PMID: 15453830
- FHL3 association with MyoD might contribute to the regulation of MyoD-dependent transcription of muscle genes, thereby influencing myogenesis. PMID: 17389685
