FIGL1 Antibody

Definition and Target of FIGL1 Antibody

The FIGL1 antibody is a polyclonal immunoglobulin developed to detect and study the Fidgetin-like 1 (FIGL1) protein, encoded by the FIGNL1 gene in humans. This antibody specifically binds to endogenous FIGL1, enabling researchers to investigate its expression, localization, and functional roles in cellular processes such as homologous recombination (HR), meiosis, and ciliogenesis .

Protein Detection and Localization

• Western Blotting: Validates FIGL1 expression in human cell lysates (e.g., HEK293T, ARPE19) .

• Immunostaining: Localizes FIGL1 to centrosomes, particularly the mother centriole, during interphase and ciliogenesis (Fig. 2B–D) .

• ELISA: Quantifies FIGL1 levels in experimental models .

Functional Studies

• Meiotic Regulation: Used to demonstrate FIGL1’s role in disassembling RAD51/DMC1 filaments during homologous recombination, preventing aberrant crossover events .

• Ciliogenesis Inhibition: Revealed FIGL1’s centrosomal accumulation and its antagonistic effect on primary cilium formation .

Role in DNA Repair and Meiosis

• FIGL1 interacts with RAD51 and DMC1 recombinases via its FRBD domain, regulating their dynamics during HR (Fig. 4C) .

• Loss of FIGL1 prolongs RAD51/DMC1 retention on chromosomes, increasing crossover frequency and aberrant recombination intermediates .

Centrosome Localization and Ciliogenesis

• Endogenous FIGL1 localizes preferentially to the mother centriole (Fig. 2C) .

• Overexpression of FIGL1 disrupts centrosomal protein localization (e.g., pericentrin, CP110) and inhibits ciliogenesis by 60% (Fig. 1A–B) .

Antagonistic Interactions

• FIGL1 counteracts BRCA2’s pro-recombination activity, balancing RAD51/DMC1 filament assembly/disassembly to ensure HR fidelity .