Customize At4g39590 Antibody

Description

Antibody Characteristics

The At4g39590 Antibody is designed for research applications in plant molecular biology. Key specifications include:

Parameter	Detail	Source
Product Code	CSB-PA635944XA01DOA
Immunogen	Recombinant Arabidopsis thaliana At4g39590 protein
Species Reactivity	Arabidopsis thaliana (Mouse-ear cress)
Applications	ELISA, Western Blot (WB)
Form	Liquid, non-conjugated
Purification Method	Antigen Affinity Purified
Storage Conditions	-20°C or -80°C; avoid repeated freeze-thaw cycles
Conjugate	Non-conjugated
Isotype	IgG
Clonality	Polyclonal

This antibody is tailored for studying the At4g39590 protein, which belongs to the galactose oxidase/kelch repeat superfamily (KEGG T00041) .

Gene and Protein Context

The AT4G39590 gene in Arabidopsis thaliana encodes a protein with potential roles in cellular processes involving kelch repeats and galactose oxidase-like domains. Key genomic and functional annotations include:

Attribute	Detail	Source
Uniprot ID	Q1PE10
Gene Family	Galactose oxidase/kelch repeat superfamily
Orthologs/Paralogs	Classified in KEGG GENOME and PGDBJ databases
Protein Domains	Kelch repeats (implicated in protein-protein interactions)

Applications and Research Potential

The antibody’s validated applications include ELISA and Western Blot, making it suitable for detecting the At4g39590 protein in plant tissues. Research gaps and potential uses include:

Epigenetic Regulation: While AT4G39590 is not directly linked to histone-modifying enzymes (e.g., Jumonji demethylases ), kelch repeat proteins may interact with chromatin-associated factors.
Stress Response: Kelch repeats are often involved in stress signaling pathways, suggesting At4g39590 could play a role in abiotic or biotic stress responses.
Protein Interaction Studies: The antibody could facilitate co-immunoprecipitation (Co-IP) to identify At4g39590 binding partners.

Limitations and Future Directions

Current data on At4g39590 Antibody is restricted to product specifications and gene annotations. Critical gaps include:

Functional Studies: No published studies directly employing this antibody to investigate At4g39590’s role in Arabidopsis.
Subcellular Localization: Immunolocalization experiments (e.g., confocal microscopy) are needed to determine the protein’s cellular compartment.
Knockout Phenotypes: Mutant analysis could reveal developmental or physiological defects linked to AT4G39590.

Comparative Analysis with Related Antibodies

While unrelated to At4g39590, insights from other plant antibodies highlight the importance of specificity and validation:

Antibody	Target	Applications	Species	Source
At4g39590 Antibody	At4g39590 protein	ELISA, WB	Arabidopsis
14-3-3 gamma Antibody (AT4B9)	14-3-3 gamma protein	ELISA, ICC, WB	Human

The At4g39590 Antibody’s plant-specific reactivity contrasts with human-targeted antibodies like AT4B9 , underscoring its niche utility in plant research.

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

At4g39590 antibody; F23K16.220F-box/kelch-repeat protein At4g39590 antibody

Target Names

At4g39590

Uniprot No.

Q1PE10

Q&A

Basic Research Questions

How to validate At4g39590 antibody specificity in Arabidopsis thaliana studies?
- Method: Perform Western blotting with A. thaliana wild-type and knockout mutants. Include isotype controls matching the antibody’s host species and clonality to rule out nonspecific binding .
- Validation metrics:
  
  Assay Type Specificity Range False Positives
  LFIA 87–96% 11.3%
  ELISA 90–97% 9.8%
What storage conditions preserve At4g39590 antibody integrity?
- Aliquot antibodies in 0.01M PBS (pH 7.4) with 50% glycerol to prevent freeze-thaw degradation . Avoid sodium azide for live-cell applications due to cytotoxicity; use 0.01% thimerosal instead .
How to optimize At4g39590 antibody dilution for immunohistochemistry?
- Titrate across a 1:100–1:1000 range using A. thaliana root cross-sections. Include antigen retrieval (e.g., citrate buffer, pH 6.0) for formalin-fixed samples .

Assay Type	Specificity Range	False Positives
LFIA	87–96%	11.3%
ELISA	90–97%	9.8%

Advanced Research Questions

How to resolve contradictory serological data in longitudinal studies?
- Approach:
  - Compare antibody kinetics across assays (e.g., Roche Elecsys vs. LFIA) .
  - Adjust for seroprevalence-dependent PPV/NPV variations:
    
    Seroprevalence PPV (IgM/IgG) NPV (IgM/IgG)
    5% 33–58% >99%
    50% 80–85% 80–85%
- Use RT-PCR-negative controls to identify cross-reactive epitopes .
What computational strategies enhance At4g39590 antibody affinity?
- Employ graph convolutional models for interface analysis (AUC = 0.83, precision = 0.89) .
- Iterative mutation optimization (e.g., Monte Carlo-based schemes) improves binding energy by 2.5-fold .
How to design experiments analyzing At4g39590-antibody structural interactions?
- Combine molecular dynamics (MD) simulations with alanine scanning mutagenesis .
- Validate using surface plasmon resonance (SPR) to quantify binding kinetics (e.g., KD < 2 nM) .

Seroprevalence	PPV (IgM/IgG)	NPV (IgM/IgG)
5%	33–58%	>99%
50%	80–85%	80–85%

Methodological Considerations

Why do some At4g39590 antibodies fail to detect native epitopes?
- Sample processing alters epitope conformation. For native-state recognition:
  - Avoid reducing/denaturing conditions .
  - Use cryopreserved tissues over FFPE samples .
How to address nonspecific binding in polyclonal At4g39590 antibodies?
- Pre-adsorb antibodies against A. thaliana lysate to remove cross-reactive immunoglobulins .
- Affinity purification with immobilized At4g39590 antigen increases specificity >90% .

Data Interpretation

What explains heterogeneous antibody responses in plant studies?
- Asymptomatic vs. symptomatic antigen exposure alters seroconversion timelines (median: 9 vs. 15 days) .
- 9.8% of subjects fail to mount detectable responses due to epitope masking .
How to validate de novo-designed At4g39590 antibodies without structural data?
- Use yeast display libraries (10^6 diversity) paired with AlphaFold2-predicted epitopes .
- Screen for subtype specificity using SPR against homologs (e.g., At4g39600) .

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At4g39590 Antibody