At5g49000 Antibody
Description
Target Protein: At5g49000
The antibody targets the protein encoded by the At5g49000 locus in Arabidopsis thaliana. Key characteristics include:
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Gene Function: The biological role of At5g49000 remains uncharacterized in published literature, though its presence in antibody catalogs suggests interest in its regulatory or structural functions .
Research Applications
While no peer-reviewed studies specifically using the At5g49000 antibody were identified, its potential applications include:
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Protein Localization: Tracking subcellular distribution via immunocytochemistry .
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Expression Profiling: Quantifying protein levels under stress conditions, akin to methods used for ASK13 in seed germination studies .
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Interaction Studies: Identifying binding partners through techniques like yeast two-hybrid screening .
Availability and Validation
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Commercial Sources: Available through Cusabio ($50 delivery fee) and PhytoAB .
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Validation Status: No independent validation data or user reviews are publicly documented .
Future Directions
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Functional Studies: Characterizing At5g49000’s role in plant development or stress responses.
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Technical Optimization: Developing protocols for Western blotting or immunofluorescence using this antibody.
Product Specs
Q&A
The At5g49000 antibody is widely used in plant molecular biology research, particularly for studying Arabidopsis thaliana proteins. Below are structured FAQs addressing both foundational and advanced methodological considerations for academic researchers.
Advanced Research Challenges
Resolving tissue-specific detection discrepancies
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Problem: Variable signal intensity in root vs. leaf extracts.
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Solution:
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Validate RNA expression correlation using qRT-PCR (ΔΔCt method)
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Test protease inhibitor cocktails for tissue-specific protein degradation
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Perform subcellular fractionation to identify localization differences
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Cross-reactivity analysis with homologous proteins
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Method:
Co-immunoprecipitation experimental design
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Critical parameters:
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Lysis buffer composition (RIPA vs. native buffers)
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Crosslinker choice (DSP vs. formaldehyde) for transient interactions
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Elution conditions for MS-compatible workflows
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Data Interpretation Framework
Experimental contradiction resolution:
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Validate antibody lot consistency via parallel testing with previous batches
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Compare results across orthogonal methods (e.g., IF vs. Western)
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Analyze post-translational modifications using Phos-tag® gels
