Customize At4g11750 Antibody

Description

Overview

The At4g11750 Antibody is a monoclonal mouse antibody specifically designed to detect the Arabidopsis thaliana protein encoded by the gene AT4G11750. This antibody targets a putative F-box/kelch-repeat protein, which is hypothesized to participate in cellular processes such as protein degradation or signaling pathways . Its primary applications include Western blot (WB) and enzyme-linked immunosorbent assay (ELISA), enabling researchers to study protein expression, localization, and interactions in plant biology .

Western Blot (WB)

The antibody is validated for detecting At4g11750 in Arabidopsis protein extracts. WB enables precise quantification and characterization of the protein’s expression levels, particularly under specific growth conditions or genetic backgrounds.

Enzyme-Linked Immunosorbent Assay (ELISA)

ELISA applications allow high-throughput screening of At4g11750 in plant tissues, facilitating studies on protein abundance, subcellular localization, or interactions with other biomolecules .

Research Findings and Potential

While direct experimental data on At4g11750’s biological role remain limited, its structural motifs suggest involvement in:

Protein Degradation: F-box proteins typically act as substrate receptors in E3 ubiquitin ligase complexes, directing target proteins for degradation via the ubiquitin-proteasome system .
Signaling Pathways: Kelch-repeat domains often mediate protein-protein interactions, potentially linking At4g11750 to stress responses or developmental signaling in Arabidopsis .

Key Advantages:

Specificity: Reactivity restricted to A. thaliana, minimizing cross-reactivity in plant model systems.
Versatility: Compatibility with WB and ELISA supports diverse experimental workflows .

Limitations and Considerations

Limitation	Implications
Lack of Functional Data	No published studies directly linking At4g11750 to specific biological pathways.
Uncharacterized Conjugates	Specific conjugate options (e.g., HRP, fluorescent tags) require experimental validation.
Limited Cross-Reactivity	Not tested in non-Arabidopsis species; unsuitable for broad plant studies.

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

At4g11750 antibody; T5C23.180Putative F-box/kelch-repeat protein At4g11750 antibody

Target Names

At4g11750

Uniprot No.

Q9T0E2

Q&A

FAQs for At4g11750 Antibody in Academic Research
This collection addresses technical challenges and methodological considerations for using the At4g11750 antibody in plant biology research. Questions are stratified by complexity, with evidence-based recommendations derived from antibody characterization literature and Arabidopsis-specific experimental data.

Advanced Research Applications

How to design a cross-species reactivity study for At4g11750 homologs?
- Experimental framework:
  1. Identify homologs using Phytozome (e.g., Brassica napus Bn4g11750-like).
  2. Express recombinant proteins for homologs in E. coli.
  3. Perform Western blot under standardized conditions (see Table 1) .
  4. Quantify cross-reactivity using densitometry and threshold at 5% signal vs. Arabidopsis target .
What computational tools can predict At4g11750 antibody-antigen binding dynamics?
- Use AlphaFold-Multimer to model antibody-epitope interactions .
- Validate predictions via surface plasmon resonance (SPR) with recombinant antigen .
- Compare computational binding affinity (ΔG) with experimental SPR data .
How to integrate At4g11750 antibody data with transcriptomic/proteomic datasets?
- Workflow:
  - Pair Western blot/immunofluorescence data with RNA-seq (e.g., tissue-specific expression from Araport).
  - Use orthogonal mass spectrometry to verify protein abundance trends .
  - Apply statistical reconciliation tools (e.g., Limma-Voom) to resolve discrepancies between mRNA and protein levels .

Troubleshooting Methodologies

Persistent high background in Western blots despite optimization
- Systematic approach:
  - Titrate antibody concentration (test 1:500 to 1:10,000 dilutions) .
  - Pre-adsorb antibody with KO lysate (1 hr, 4°C) to remove nonspecific binders .
  - Switch to a chemiluminescent substrate with higher signal-to-noise ratio (e.g., SuperSignal West Femto) .
Discrepancies between antibody-based and CRISPR-edited phenotypic data
- Investigate off-target effects via:
  - RNA-seq of KO lines to confirm no compensatory gene regulation.
  - Dual antibody validation (e.g., At4g11750 and a co-expressed marker) .
- Reconcile data using a Bayesian probability model to weigh evidence from both methods .

Ethical and Reporting Standards

Always disclose antibody lot numbers, dilution ratios, and validation data in publications .
Deposit raw imaging data in repositories like BioImage Archive to enable independent verification .

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At4g11750 Antibody