FKBP17-2 Antibody
Description
Role in Plant Immunity
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Cotton bollworm interaction: In Gossypium hirsutum (cotton), FKBP17-2 mediates resistance against Helicoverpa armigera (cotton bollworm). Knockdown of GhFKBP17-2 reduces ER stress sensor gene expression (e.g., GhEFR, GhbZIP23), impairing plant defense mechanisms .
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PPIase activity: Enzymatic assays confirm that GhFKBP17-2’s PPIase activity is essential for regulating immune responses. Structural domain deletions (e.g., △1) abolish this activity .
Viral Pathogenesis
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Citrus Tristeza Virus (CTV): FKBP17-2 interacts with CTV’s p23 and coat proteins, facilitating viral replication. Knockdown of FKBP17-2 in Nicotiana benthamiana reduces CTV accumulation, indicating a proviral role .
Stress Response Regulation
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ER stress modulation: FKBP17-2 contributes to ER stress signaling by upregulating stress sensors like GhWRKY33 and GhBiP5, which are critical for managing unfolded protein responses .
Table 1: FKBP17-2 Homologs in Arabidopsis thaliana
| Gene ID | Protein Name | Molecular Weight (kDa) | PPIase Activity | Localization |
|---|---|---|---|---|
| AT1G18170.1 | FKBP17-2 | 16.9 | Confirmed | ER-associated |
| AT4G19830.1 | FKBP17-1 | 16.7 | Not confirmed | Undetermined |
Table 2: Functional Impact of FKBP17-2 Knockdown
| Organism | Phenotype Observed | Key Downregulated Genes | Citation |
|---|---|---|---|
| G. hirsutum | Reduced insect resistance, impaired ER stress | GhEFR, GhbZIP23, GhBiP5 | |
| N. benthamiana | Decreased CTV replication | — |
Applications of FKBP17-2 Antibody
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Immunoblotting: Used to detect FKBP17-2 expression in transgenic plants (e.g., cotton lines overexpressing GhFKBP17-2) .
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Subcellular localization: Antibody-based imaging confirms ER localization in plant cells .
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Functional studies: Facilitates research on FKBP17-2’s role in viral pathogenesis and stress responses .
Future Directions
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Therapeutic potential: Targeting FKBP17-2 could enhance crop resistance to pathogens or modulate ER stress in biomedical contexts.
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Mechanistic studies: Further structural analysis of FKBP17-2-ligand interactions may reveal novel regulatory pathways.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Q&A
FAQs for FKBP17-2 Antibody in Academic Research
Advanced Research Questions
How can I resolve contradictions in FKBP17-2 subcellular localization across studies?
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Methodological Answer:
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Technical Variables: Differences in fixation methods (e.g., paraformaldehyde vs. methanol) or antigen retrieval (e.g., citrate buffer pH 6.0 vs. TE buffer pH 9.0) can alter staining patterns .
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Biological Context: FKBP17-2 may localize differently in stress conditions (e.g., ER stress) or cell types (e.g., neurons vs. epithelial cells) .
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Validation: Combine IF with subcellular fractionation/Western blotting to corroborate localization .
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What strategies mitigate cross-reactivity between FKBP17-2 and other FKBP isoforms?
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Methodological Answer:
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Epitope Mapping: Use antibodies targeting unique regions (e.g., N-terminal vs. C-terminal domains) verified by alignment tools like Clustal Omega .
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Competitive Peptide Blocking: Pre-incubate antibody with excess immunogen peptide; loss of signal confirms specificity .
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Multiplex Knockdown: Co-silence FKBP17-2 and homologs (e.g., FKBP12) to isolate isoform-specific functions .
Example: A study on FKBP10 used antigen retrieval with TE buffer pH 9.0 to distinguish it from FKBP65 in ovarian cancer .
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How should I interpret discrepancies in FKBP17-2 expression data between Western blot and IHC?
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Methodological Answer:
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Post-Translational Modifications (PTMs): FKBP17-2 may undergo phosphorylation or ubiquitination, altering electrophoretic mobility .
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Antibody Sensitivity: IHC may detect low-abundance membrane-bound forms, while Western blot requires higher protein input .
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Quantitative Calibration: Normalize IHC signals using H-scores and Western blot signals via housekeeping proteins (e.g., GAPDH) .
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What functional assays integrate FKBP17-2 antibody data to study cellular roles?
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Methodological Answer:
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Co-Immunoprecipitation (Co-IP): Identify FKBP17-2 interaction partners (e.g., TGF-β receptors) using validated antibodies .
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Phenotypic Rescue: Express FKBP17-2 in KO cells and monitor recovery of cellular processes (e.g., protein folding) .
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High-Content Screening: Combine IF with automated analysis to quantify FKBP17-2 dynamics under drug treatment .
Example: FKBP12’s role in TGFBR1 regulation was confirmed via Co-IP and MDM2 degradation assays .
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