FKBP42 Antibody
Description
Target Protein Overview
FKBP42/TWD1 is a 42 kDa protein (UniProt: NP_188801.2) localized to the endoplasmic reticulum (ER) and plasma membrane . It functions as a chaperone for ATP-BINDING CASSETTE (ABC) transporters and modulates brassinosteroid (BR) signaling . Key features include:
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Domains: FK506-binding domain, tetratricopeptide repeat (TPR), calmodulin-binding domain
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Interactions: Binds HSP90.1, calmodulin, and ABCB transporters
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Biological roles:
Antibody Applications
The FKBP42 antibody (e.g., MyBioSource MBS9011012) is a rabbit polyclonal antibody used in:
Role in Brassinosteroid Signaling
FKBP42/TWD1 is essential for BR signal transduction:
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Enhances BRI1-BAK1 kinase complex activation but does not affect FLS2-mediated immunity
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Mechanism: Directly interacts with BRI1/BAK1 kinase domains to stabilize receptor complexes
Regulation of Auxin Transport
FKBP42/TWD1 governs ABCB transporter trafficking:
Dual Role in Hormone-Immunity Crosstalk
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twd1 mutants show enhanced flagellin responses (MAPK activation, pathogen gene expression)
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BR signaling deficiency in twd1 indirectly amplifies immune responses
Technical Considerations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
• **Modulation of Vacuolar Transport:** TWD1 influences the uptake of MRP substrates into the vacuole, a cellular organelle involved in detoxification and storage. It has been shown to reduce the uptake of metolachlor-GS (MOC-GS), a herbicide, and enhance the uptake of 17-beta-estradiol 17-(beta-D-glucuronide) (E(2)17betaG), a steroid hormone conjugate.
• **Regulation of Cell Growth and Development:** TWD1 plays a role in regulating cell elongation and orientation. It functions as a positive regulator of PGP1-mediated auxin transport, a key hormone responsible for plant growth and development.
• **Drug Modulation of PGP1 Efflux Activity:** TWD1 interacts with PGP1, a drug efflux pump, influencing its activity. Notably, interaction with NPA or flavonol quercetin prevents TWD1 from physically and functionally interacting with PGP1, thereby modulating drug efflux.
• **Localization of Auxin-Related ABCB Transporters:** TWD1 is essential for the proper localization of auxin-related ABCB transporters, a family of membrane proteins involved in auxin transport.
• **Brassinosteroid Signaling Pathway:** TWD1 participates in the brassinosteroid (BR) signaling pathway, a complex network of signaling molecules that regulate plant growth and development.
- TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics PMID: 27053424
- FKBP42/TWD1 plays a specific role in the activation of BRI1 receptor kinase PMID: 26808213
- TWISTED DWARF 1 Associates with BRASSINOSTEROID-INSENSITIVE 1 to Regulate Early Events of the Brassinosteroid Signaling Pathway PMID: 26802250
- TWD1 immunophilin affects auxin transport and root growth by affecting B-group ABC transporters. PMID: 20971896
- the X-ray structure of the cytoplasmic portion of FKBP42 comprising both the FKBP-like domain and the TPR domain at 2.85 A resolution PMID: 17045295
