FLA1 Antibody

Introduction to FLA1 Antibody

The FLA1 antibody targets the FLAD1 (FAD1 flavin adenine dinucleotide synthetase homolog) protein, a critical enzyme involved in converting riboflavin (vitamin B2) into flavin adenine dinucleotide (FAD), a redox cofactor essential for cellular respiration, energy production, and metabolic pathways . FLAD1 is conserved across eukaryotes and prokaryotes, with human FLAD1 localized in the cytoplasm and requiring magnesium as a cofactor . While "FLA1" may occasionally refer to unrelated proteins (e.g., flagellin in bacterial studies ), this article focuses on FLAD1-targeting antibodies due to their broader scientific relevance.

Key Applications of FLAD1 Antibodies

FLAD1 antibodies are widely used in research to study FAD biosynthesis, metabolic disorders, and cancer biology. Common applications include:

FLAD1 in Metabolic Disorders

FLAD1 deficiency is linked to lipid storage myopathy and multiple acyl-CoA dehydrogenase deficiency (MADD), disorders characterized by impaired fatty acid oxidation. Studies using FLAD1 antibodies have identified mutations in the FLAD1 gene that disrupt FAD synthesis, leading to metabolic dysfunction .

Role in Cancer Biology

FLAD1 is implicated in cancers due to its chromosomal location (1q21.3), a region frequently altered in malignancies like multiple myeloma and melanoma . FLAD1 antibodies enable researchers to track its expression in tumor microenvironments, revealing potential therapeutic targets.

Table 2: Recommended Dilutions

Key Notes:

• Cross-reactivity with non-target species (e.g., rat, mouse) varies by product .

• Storage at -20°C or -80°C preserves antibody integrity .

Challenges and Considerations

• Glycosylation Variability: FLAD1’s glycosylation patterns differ across cell types, affecting antibody binding .

• Isoforms: Five splice variants of FLAD1 exist, necessitating isoform-specific validation .