FLS1 Antibody
Description
Introduction to FLS1 Antibody
The FLS1 Antibody (SKU: AT5G08640) is a primary antibody targeting Flavonol Synthase/Flavanone 3-Hydroxylase, an enzyme critical in flavonoid biosynthesis. This antibody is designed for research applications, particularly in studying plant metabolic pathways, enzymatic activity, and gene expression related to flavonol synthesis.
Target and Function
FLS1 catalyzes the conversion of dihydroflavonols to flavonols, a key step in the flavonoid biosynthesis pathway. Flavonols are secondary metabolites with roles in plant defense, UV protection, and antioxidant activity. The antibody is likely used to detect FLS1 protein expression in plant tissues, enabling researchers to study:
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Enzyme localization (e.g., in leaves, roots, or flowers).
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Gene expression regulation under environmental stressors (e.g., UV light, pathogens).
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Metabolic flux analysis in engineered or mutant plant lines.
Applications in Research
While specific research findings are not detailed in publicly available sources, the antibody’s utility can be inferred from its target’s biological role:
| Application Area | Potential Use Case |
|---|---|
| Plant Metabolomics | Quantifying FLS1 protein levels in response to abiotic/biotic stressors. |
| Gene Editing | Validating CRISPR/Cas9-mediated knockout or overexpression of FLS1 in plant models. |
| Flavonoid Engineering | Tracking FLS1 activity in bioengineered crops with enhanced antioxidant production. |
Current Availability and Future Directions
As of the latest available data, the FLS1 Antibody is temporarily out of stock but in production. Researchers are advised to contact the supplier (e.g., PhytoAb) for custom orders or updates. Potential future applications may include:
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Epitope-specific studies to map FLS1’s catalytic domains.
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Cross-reactivity testing with homologous enzymes in other plant species.
Limitations and Considerations
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Research Gaps: No peer-reviewed studies or experimental data (e.g., Western blot, immunohistochemistry results) are publicly available for this antibody.
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Target Specificity: Confirmation of cross-reactivity with non-target proteins is essential for experimental validation.
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Technical Support: Researchers are encouraged to consult the supplier for protocols or troubleshooting.
Product Specs
Target Background
Q&A
Here’s a structured collection of FAQs for FLS1 antibody research, synthesized from interdisciplinary antibody research principles and methodologies:
Advanced Research Questions
How to resolve contradictory FLS1 localization data between IHC and Western blot?
Conduct systematic troubleshooting:
| Discrepancy Source | Diagnostic Approach | Resolution Strategy |
|---|---|---|
| Epitope Accessibility | Limited antigen retrieval in IHC | Test multiple retrieval buffers (citrate vs. EDTA) |
| Post-Translational Modifications | Phos-tag/Western analysis | Use modification-specific antibodies |
| Antibody Batch Variability | Parallel testing of multiple lots | Establish internal reference standard |
What computational strategies improve FLS1 antibody-antigen binding predictions?
Implement hybrid physics-ML approaches:
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Force-guided diffusion sampling: Integrate molecular dynamics (MD)-derived energy terms into generative models:
Where weights () balance physical and learned potentials -
Interface-focused docking: Use RosettaAntibody with FLS1 paratope constraints
How to design a longitudinal study assessing FLS1 dynamics in chronic inflammation?
Adopt a multi-omics framework:
| Timepoint | Data Layer | Analytical Integration |
|---|---|---|
| Baseline | scRNA-seq | Pseudotime trajectory analysis |
| 6 Months | CyTOF (30+ markers) | Network correlation to FLS1+ subset |
| 12 Months | Olink Proteomics | LASSO regression for biomarker linkage |
Methodological Innovation Challenges
What strategies mitigate batch effects in multi-center FLS1 studies?
Implement harmonized protocols:
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Pre-analytical: Standardize fixation time (±15 mins) and temperature
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Analytical: Use reference standards (e.g., HER2 IHC controls) across sites
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Computational: Apply ComBat harmonization with variance stabilization:
How to validate FLS1 antibody performance in 3D organoid models?
Triangulate methods:
