FMO2 Antibody

Introduction to FMO2 Antibody

The FMO2 antibody is a polyclonal rabbit IgG reagent designed to detect flavin-containing monooxygenase 2 (FMO2), a 50–60 kDa enzyme involved in oxidative protein folding and cellular stress responses . This antibody is critical for identifying FMO2 expression in research models, enabling insights into its roles in cardiac fibrosis, renal injury, cancer progression, and endoplasmic reticulum (ER) stress modulation . Validated across human and mouse tissues, it supports applications including Western blot (WB), immunohistochemistry (IHC), and ELISA .

Key Attributes of FMO2 Antibodies

FMO2 antibodies exhibit consistent detection of FMO2 isoforms across species, with observed molecular weights of 50–60 kDa . Proteintech’s antibody utilizes a fusion protein immunogen, while Sigma’s offering is validated through the Human Protein Atlas project, which includes subcellular localization data .

Cardiac Fibrosis and Injury

• Mechanism: FMO2 inhibits TGF-β/SMAD2/3 signaling in cardiac fibroblasts, reducing post-myocardial infarction (MI) fibrosis .

• Therapeutic Evidence:

  • Lentiviral FMO2 delivery in rats improved ejection fraction by 22% and reduced infarct size by 35% post-MI .

  • In nonhuman primates, FMO2 gene therapy attenuated left ventricular dilation and collagen deposition .

Renal Fibrosis and Ischemia-Reperfusion Injury

• Functional Role: FMO2 overexpression in renal tubular cells suppressed TGF-β–induced SMAD2/3 activation and reduced immune cell infiltration (e.g., neutrophils and macrophages) by 40–60% .

• Outcome: FMO2 expression correlated with a 50% decrease in pro-inflammatory cytokines (IL-6, CXCL10) and mitigated renal fibrosis in murine models .

Gastric Cancer (GC) Prognosis

• Expression Patterns:

  FMO Isoform	GC vs. Normal	Prognostic Relevance
  FMO1	Overexpressed	Poor survival
  FMO2	Under-expressed	Tumor suppression

• Immune Modulation: FMO2 expression inversely correlates with M0 macrophages (−0.32, P<0.01) and activated dendritic cells (−0.28, P<0.05), suggesting immune microenvironment regulation .

Validation and Reliability Data

• Human Protein Atlas: FMO2 antibodies validated in 44 normal and 20 cancer tissues, confirming specificity in lung, kidney, and heart .

• Quality Control:

  • Proteintech’s antibody shows >95% purity via antigen-affinity chromatography .

  • Sigma’s antibody tested against 364 recombinant proteins, ensuring minimal cross-reactivity .

Optimal Conditions for FMO2 Detection

Titration is recommended for each experimental system to balance signal-to-noise ratios .