FMP33 Antibody

Biological Context of FMP33

FMP33 is a mitochondrial membrane protein in S. cerevisiae involved in energy generation and oxidative stress responses . Its expression is tightly regulated under stress conditions:

• Upregulation: Observed in response to Hog1 pathway activation (fold change: +2.67) .

• Function: Associates with mitochondrial membrane integrity and electron transport chain efficiency.

Research Applications

The FMP33 antibody is primarily used in yeast biology studies to:

• Track Mitochondrial Dynamics: Monitor FMP33 localization and abundance in mitochondrial membranes.

• Study Stress Responses: Investigate FMP33’s role in oxidative stress, heat shock, and Hog1 signaling .

• Protein-Protein Interactions: Identify binding partners via co-immunoprecipitation (Co-IP).

Example Use Case:
In a 2015 study, FMP33 was found to co-localize with Hog1 signaling components, suggesting its role in stress adaptation . The antibody enabled detection of FMP33 upregulation in Hog1-activated cells (fold change: +2.67).

Technical Performance

The antibody demonstrates robust performance in standard assays:

• Western Blotting: Detects FMP33 at ~30 kDa in yeast lysates (SDS-PAGE resolution).

• ELISA: Exhibits high specificity for recombinant FMP33 (IC50: 0.5–1.0 μg/mL).

Potential in Yeast Research

FMP33’s role in mitochondrial function positions it as a critical marker for studies on:

• Mitochondrial Diseases: Yeast models of human mitochondrial disorders.

• Bioenergy Research: Optimization of yeast-based biofuel production.

• Stress Tolerance: Engineering yeast for industrial processes under stress conditions.

Citations and References

- Creative Biolabs. Mouse Anti-Yeast FMP33 Antibody.
- Yeast genome-wide expression study (2015).
- Saccharomyces Genome Database (SGD). FMP33.