At3g61080 Antibody

Research Applications

The At3g61080 Antibody is utilized in diverse experimental contexts:

• Gene Expression Analysis: Quantification of AT3G61080 mRNA/protein levels via immunoblotting or immunofluorescence .

• Epigenetic Studies: Investigation of gene silencing and paramutation in polyploid Arabidopsis lines .

• Cellular Localization: Tracking protein distribution in tissues like gynoecia, septa, or ovules .

Key Findings:

• In ntt stk double mutants, AT3G61080 expression is reduced, indicating regulation by NO TRANSMITTING TRACT (NTT) and STK proteins .

• The antibody aids in studying cell wall metabolism and transmitting tract development in plant reproductive tissues .

Gene Regulation

• NTT-STK Interaction: AT3G61080 is part of a core coexpression network regulated by NTT and STK. ChIP-qPCR experiments confirmed promoter binding of STK to AT3G61080, suggesting direct transcriptional control .

• Polyploidy Studies: In autotetraploid Arabidopsis, AT3G61080 expression patterns were analyzed alongside epigenetic changes during paramutation .

Methodological Utility

• Immunoblotting: Used to validate protein knockdown in mutants .

• Immunolocalization: Combined with LM21 antibody (mannan detection) to study cell wall polysaccharide dynamics .

Comparative Analysis with Related Antibodies

Future Directions

The At3g61080 Antibody remains critical for:

1. Elucidating metabolic pathways in carbohydrate processing.

2. Deciphering epigenetic interactions in polyploid genomes.

3. Validating CRISPR-edited mutants in Arabidopsis functional studies.