FOLB2 Antibody

Introduction to FOLR2 Antibody

FOLR2 (Folate Receptor Beta) is a glycoprotein critical for folate transport and cellular metabolism. The FOLR2 antibody targets this receptor, which is overexpressed in tumor-associated macrophages (TAMs) and certain cancers (e.g., gliomas, breast, lung, and colon carcinomas) . While the query specifies "FOLB2 Antibody," extensive search results indicate a likely typographical error, as no references to "FOLB2" exist in scientific literature. All relevant data pertains to FOLR2, a well-characterized protein. Below is a detailed analysis of FOLR2 antibodies, their applications, and research findings.

FOLR2 Antibody Characteristics

Immunohistochemistry (IHC)

FOLR2 antibodies enable visualization of receptor expression in tissues. For example, the CL10 antibody detects FRbeta in tumor-associated macrophages, aiding cancer diagnostics . Optimal dilutions must be experimentally determined .

Western Blot (WB)

• CL10: Detects 29 kDa FOLR2 in mouse heart, lung, pancreas, testis, and rat ovary .

• M04574-1: Identifies 29 kDa bands in human spleen and placenta lysates (1:1000–1:2000 dilution) .

Immunocytochemistry (ICC)

The MAB5697 antibody localizes FOLR2 to human neutrophil surfaces and cytoplasm, confirming its role in immune cell function .

Role in Cancer and Macrophages

FOLR2 is selectively expressed on activated macrophages in tumor microenvironments, making it a therapeutic target. Antibodies like CL10 bind FRbeta, enabling toxin delivery to TAMs while sparing healthy macrophages .

Antibody Validation

• Specificity: CL10 and MAB5697 show no cross-reactivity with FOLR1 or other folate receptors .

• KO Cell Lines: Recombinant antibodies outperform polyclonal counterparts in specificity and reproducibility .

Table 1: Antibody Reactivity and Applications

Table 2: Western Blot Results