FOPNL Human

FGFR1OP N-terminal like Human Recombinant
Shipped with Ice Packs
In Stock

Description

FOPNL Human Recombinant produced in E. coli is a single polypeptide chain containing 194 amino acids (1-174) and having a molecular mass of 21.9 kDa.
FOPNL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
FOPNL (FGFR1OP N-terminal like) belongs to the FGFR1OP family and possesses a LisH domain. This protein plays a role in cilia formation. FOPNL is widely distributed in various tissues, including the brain, heart, kidney, liver, lung, skeletal muscle, placenta, and intestine.
Description
Recombinant human FOPNL, produced in E. coli, is a single polypeptide chain consisting of 194 amino acids (residues 1-174) with a molecular weight of 21.9 kDa. It includes a 20 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The FOPNL solution (0.5mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freezing and thawing.
Purity
Purity exceeds 85% as assessed by SDS-PAGE analysis.
Synonyms
FGFR1OP N-terminal like-like protein, FOP-related protein of 20 kDa, pluripotent embryonic stem cell-related protein, lisH domain-containing protein C16orf63, chromosome 16 open reading frame 63, FGFR1OP N-terminal, PHSECRG2, C16orf63, DKFZp686N1651, FLJ31153, FOR20.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MATVAELKAV LKDTLEKKGV LGHLKARIRA EVFNALDDDR EPRPSLSHEN LLINELIREY LEFNKYKYTA SVLIAESGQP VVPLDRQFLI HELNAFEESK DNTIPLLYGI LAHFLRGTKD GIQNAFLKGP SLQPSDPSLG RQPSRRKPMD DHLRKEEQKS TNIEDLHVSQ AVNR

Q&A

What are the main types of FOPNL systems currently used in research studies?

When designing FOPNL studies, researchers must consider that these systems vary considerably in their:

Additionally, some regions have developed hybrid or modified formats that combine elements from these standard approaches, requiring careful documentation of the specific FOPNL characteristics being tested.

How do researchers effectively measure consumer understanding of FOPNL systems?

Measurement of consumer understanding typically employs both subjective and objective assessment methods. According to recent methodological frameworks:

  • Objective understanding is measured through tasks requiring participants to correctly identify healthier products, rank products by nutritional quality, or explain the meaning of specific label elements .

  • Subjective understanding assesses participants' perceived comprehension of labels using Likert scales for factors such as clarity, comprehensibility, and cognitive workload .

  • Comparative assessment involves showing participants multiple FOPNL formats and evaluating their preference, perceived usefulness, and comprehension across formats .

The most robust studies combine these approaches, often using mock packages with fictional brands to control for brand loyalty effects and randomizing the order of presentation to minimize order effects .

What outcome measures are most relevant for FOPNL effectiveness research?

Research primarily examines four categories of outcome measures:

  • Attentional measures: Noticeability (what participants first notice on packaging), visual attention (measured through self-report or eye-tracking)

  • Cognitive processing measures:

    • Perceived cognitive workload

    • Speed of information processing

    • Legibility and comprehension

  • Behavioral intention measures:

    • Purchase intentions

    • Product choice in simulated environments

    • Stated willingness to change consumption patterns

  • Actual behavioral measures:

    • Sales data from retail environments

    • Changes in purchasing patterns over time

    • Nutritional quality of shopping baskets

The literature indicates that evaluative labels are often processed faster than purely informative ones, with differential effects observed across consumer segments .

How should researchers design randomized experiments for FOPNL evaluation?

Robust FOPNL experiments require careful attention to:

  • Participant randomization: Stratified randomization ensures balanced representation across demographic variables. In multi-centric studies, equal distribution of participants from different income strata (high, medium, low economic, and rural areas) is recommended .

  • FOPNL allocation: Participants should be randomly assigned to FOPNL formats to prevent selection bias. For example, in a recent study with 500 participants per location, 25 participants from each income group were allocated to each of the five FOPNL formats being tested .

  • Stimulus randomization: The order of product presentation should be randomized to prevent order effects. This is particularly important when showing multiple variants of products with different nutritional profiles .

  • Blinding procedures: Where possible, researchers and interviewers should be blinded to hypotheses to prevent experimenter bias.

  • Cross-over designs: For within-subject comparisons of multiple FOPNL formats, sufficient washout periods between exposures are necessary.

How can researchers address sociodemographic variables in FOPNL studies?

Sociodemographic factors significantly influence FOPNL effectiveness. Methodologically sound approaches include:

  • Stratified sampling: Ensuring representation across age, education, income, and geographical location (urban/rural) .

  • Tailored assessment: Adapting testing protocols for different literacy levels while maintaining measurement equivalence.

  • Statistical control: Including sociodemographic variables as covariates in analyses of FOPNL effectiveness.

  • Moderation analysis: Examining how sociodemographic factors moderate the relationship between FOPNL exposure and outcomes.

  • Subgroup analysis: Analyzing effects separately for vulnerable populations (e.g., adolescents, those with non-communicable diseases) .

Research indicates that label reading practices vary significantly by education level, income, and health status, with those reporting non-communicable diseases showing different patterns of engagement with nutritional information .

What methodological approaches can address cross-cultural differences in FOPNL interpretation?

Cross-cultural FOPNL research requires:

  • Cultural adaptation: Adjusting visual elements, colors, and symbols to local cultural contexts while maintaining conceptual equivalence.

  • Translation protocols: Using forward-backward translation for all research instruments, with expert panel review to ensure cultural appropriateness.

  • Mixed-methods approaches: Combining quantitative measures with qualitative exploration of cultural interpretations.

  • Measurement invariance testing: Statistically confirming that measurement instruments function equivalently across cultural groups.

  • Multi-centric design: Including diverse geographical locations to capture cultural variation, such as the approach used in recent Indian research spanning multiple regions .

How should mock packages be designed for valid FOPNL testing?

Mock package design requires careful attention to experimental control while maintaining ecological validity. Best practices include:

  • Fictional branding: Using fictional brands (e.g., "Delish") to eliminate brand loyalty effects .

  • Nutritional variation: Creating multiple variants (typically 5) representing a spectrum from "healthiest to least healthy" products .

  • Visual consistency: Maintaining identical packaging elements except for the FOPNL format and nutritional information being tested.

  • Realistic design: Ensuring packages resemble actual market products in size, format, and information layout.

  • Pre-testing: Validating mock packages with expert panels and pilot consumer groups before full study implementation .

The mock packages should be created in sets that allow for direct comparison of how different FOPNL formats communicate the same nutritional information across the health spectrum.

What questionnaire development process ensures valid FOPNL assessment?

Research indicates a systematic approach to questionnaire development:

  • Initial item pool: Creating a comprehensive pool of potential questions (e.g., 50 initial items) .

  • Expert panel review: Having multidisciplinary experts (psychology, health communication, nutrition, public health) evaluate content validity .

  • Iterative refinement: Revising through investigator consensus to eliminate redundancy and improve clarity .

  • Pilot testing: Field-testing with a representative sample (typically n=100) across all study locations .

  • Final revision: Making adjustments based on pilot findings, including rewording difficult questions, removing redundancies, and optimizing question sequence .

  • Standardized training: Training field staff in standardized administration to ensure measurement consistency across sites .

This process resulted in validated instruments that effectively measure comparative acceptability, objective understanding, and purchase intentions across different FOPNL formats .

How should researchers analyze data comparing multiple FOPNL formats?

Comparative FOPNL analysis requires sophisticated analytical approaches:

  • Preferential ranking analysis: Analyzing participant rankings of FOPNL formats (from most to least preferred) using appropriate non-parametric statistics .

  • Likert scale analysis: For questions assessing likeability, attractiveness, and perceived cognitive workload of each format on 5-point scales .

  • Paired comparisons: Directly comparing participant responses across formats on key dimensions:

    • Noticeability

    • Legibility

    • Comprehension and cognitive workload

    • Speed of processing

    • Informativeness

  • Between-group analysis: For randomized components, comparing outcomes between participant groups assigned to different FOPNL formats using appropriate statistical tests.

  • Demographic stratification: Analyzing format effectiveness across different sociodemographic segments.

What methodological challenges exist in interpreting FOPNL study results?

Researchers should address several interpretative challenges:

  • Distinction between understanding and behavior: Objective understanding of a FOPNL format doesn't necessarily translate to behavioral change. Studies should measure both understanding and behavioral intentions/outcomes .

  • Self-report limitations: Purchase intentions may not align with actual purchasing behavior. Triangulation with observational or sales data strengthens validity.

  • Novelty effects: New FOPNL formats may gain attention initially due to novelty rather than inherent effectiveness. Longitudinal designs can address this limitation.

  • Context dependency: FOPNL effectiveness varies with product category, shopping context, and consumer motivation. These contextual factors should be systematically varied or controlled.

  • Ecological validity: Laboratory-based studies may not generalize to real-world shopping environments. Mixed-method designs combining controlled experiments with field studies offer stronger evidence.

How should researchers approach the analysis of consumer attention to FOPNL in the context of competing package elements?

Analysis of consumer attention requires specialized methods:

  • First-attention analysis: Recording the first 3-4 elements that participants notice on food packaging to determine if FOPNL naturally captures attention .

  • Comparative salience: Measuring which package elements (brand name, product image, FOPNL, claims) most consistently attract consumer attention.

  • Information hierarchy: Analyzing how attention to FOPNL relates to attention to other nutritional information on packaging.

  • Attention duration: Quantifying how long consumers attend to FOPNL compared to other packaging elements.

  • Integration with eye-tracking: Complementing self-report with objective eye-tracking data when available.

Research shows that consumers often attend to multiple elements before noticing FOPNL, highlighting the importance of label design that increases visual salience .

What standardized training approaches ensure consistent data collection in multi-centric FOPNL studies?

Multi-centric studies require rigorous training protocols:

  • Centralized training: Conducting training programs for field investigators to standardize questionnaire administration .

  • Individual coaching: Providing one-on-one sessions on questionnaire administration with project assistants from each study location .

  • Procedure manuals: Developing detailed manuals to guide staff on interview conduct and data collection procedures .

  • Field supervision: Implementing supervision by principal investigators and co-investigators to assess survey modalities and ensure quality control .

  • Ongoing calibration: Conducting regular check-ins to address emergent issues and maintain consistency across sites.

These approaches minimize measurement error and ensure comparability of data across diverse research locations.

How can researchers integrate evolving digital technologies into FOPNL research?

Digital approaches offer new opportunities for FOPNL research:

  • Virtual reality shopping: Simulating grocery environments to observe FOPNL influence on product selection in controlled but realistic settings.

  • Mobile eye-tracking: Using wearable eye-tracking devices to study FOPNL attention in actual retail environments.

  • Smartphone-based ecological momentary assessment: Capturing real-time decision-making and FOPNL influence at point of purchase.

  • Digital cohorts: Following consumers longitudinally through digital platforms to measure long-term FOPNL effects.

  • Machine learning analysis: Using advanced algorithms to identify patterns in consumer responses to different FOPNL formats across large datasets.

These approaches can complement traditional methods to provide more ecologically valid and detailed insights into FOPNL effectiveness.

Product Science Overview

Introduction

FGFR1OP N-terminal like (FOPNL) is a protein that belongs to the FGFR1OP family. It is known for its role in the biogenesis of cilia, which are hair-like structures on the surface of cells that play crucial roles in cell movement and signaling . FOPNL is extensively expressed in various tissues, including the brain, heart, kidney, liver, lung, skeletal muscle, placenta, and intestine .

Gene and Protein Structure

The FGFR1OP gene encodes a largely hydrophilic protein that is postulated to be a member of the leucine-rich protein family . The gene is located on chromosome 6 in humans and chromosome 17 in mice . The protein contains one LisH domain, which is involved in protein-protein interactions and is essential for the protein’s function in cilia biogenesis .

Function and Biological Role

FGFR1OP N-terminal like protein plays a significant role in normal cell proliferation and differentiation, particularly in the erythroid lineage . It is involved in various cellular processes, including the regulation of protein kinase activity, cell migration, cell growth, and the organization of cell projections . The protein is also crucial for the G2/M transition of the mitotic cell cycle and the anchoring of microtubules .

Clinical Significance

A chromosomal translocation involving the FGFR1OP gene and the fibroblast growth factor receptor 1 (FGFR1) gene has been identified in cases of myeloproliferative disorder . This translocation results in a chimeric protein that contains the N-terminal leucine-rich region of FGFR1OP fused to the catalytic domain of FGFR1 . This chimeric protein is thought to contribute to the abnormal proliferation and differentiation observed in myeloproliferative disorders .

Recombinant Production

FGFR1OP N-terminal like (Human Recombinant) is produced in Escherichia coli (E. coli) as a single polypeptide chain containing 194 amino acids, with a molecular mass of 21.9 kDa . The recombinant protein is fused to a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques . The protein is available in various quantities and is used primarily for laboratory research purposes .

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