FOX4 Antibody
Description
Applications in Research
Key Research Findings
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Aging: FOXO4-DRI (a competitive inhibitor) reduces senescent cell burden in aged mice, restoring tissue function (e.g., kidney homeostasis) .
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Cancer: FOXO4 overexpression inhibits colorectal cancer metastasis by enhancing APC2/β-catenin signaling .
Applications in Research
| Application | Validation Data | Sources |
|---|---|---|
| Western Blot | Detects recombinant FOXP4 at ~75 kDa in transfected cell lines . | |
| Immunocytochemistry (ICC) | Nuclear staining in human tonsil and HepG2 cells . |
Key Research Findings
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Neurodevelopment: FOXP4 deficiency disrupts neuronal circuit formation in animal models .
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Cancer: FOXP4 is overexpressed in hepatocellular carcinoma, correlating with tumor progression .
Comparative Analysis of FOXO4 and FOXP4 Antibodies
FOXO4 Antibodies
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Proteintech 21535-1-AP: Validated for WB, IHC, and ELISA; reacts with human, mouse, and rat samples .
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Rockland 600-401-BG7: Targets N-terminal epitopes; used in IF and IHC .
FOXP4 Antibodies
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Abcam ab251688: Rabbit polyclonal antibody for IHC-P, WB, and ICC/IF .
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R&D Systems AF6854: Sheep polyclonal antibody validated in WB for human samples .
Technical Considerations
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Specificity: FOXO4 antibodies may cross-react with FOXO1/3 due to structural homology. Epitope mapping (e.g., N-terminal vs. C-terminal) is critical .
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Buffer Compatibility: Glycerol-based storage buffers (e.g., 40–50%) enhance long-term stability .
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Validation: KO cell lines or peptide blocking assays are recommended to confirm specificity .
Emerging Therapeutic Applications
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
