FOXB2 Antibody
Description
Overview of FOXB2 and Its Antibody
FOXB2 (Forkhead Box Protein B2) regulates gene expression involved in cell proliferation, differentiation, and developmental processes . The FOXB2 antibody (e.g., PACO17881) is a polyclonal reagent generated in rabbits, targeting a synthetic peptide of human FOXB2. It enables precise detection in experimental models, particularly in human tissues .
Immunohistochemistry (IHC)
The FOXB2 antibody has been used to identify FOXB2 expression in paraffin-embedded cancer tissues. For example:
-
Lung Cancer: Distinct FOXB2 staining was observed in tumor cells at a dilution of 1:5, with signal specificity confirmed by peptide blockade .
-
Gastric Cancer: Similar reactivity patterns were noted, supporting its utility in cancer biomarker studies .
Functional Studies
FOXB2 is implicated in transcriptional regulation of genes affecting cell fate and immune responses. Dysregulation of FOXB2 is linked to cancers and developmental disorders, making this antibody vital for mechanistic studies .
Associated Assays and Experimental Data
The FOXB2 Transcription Factor Activity Assay (TFAB00107) complements antibody-based studies by quantifying FOXB2’s DNA-binding activity in nuclear lysates :
| Parameter | Detail |
|---|---|
| Target | FOXB2 |
| Reactivity | Human, Mouse, Rat |
| Sample Types | Nuclear or cell lysates |
| Applications | Gene regulation studies, drug screening |
This assay detects FOXB2’s role in repressing immune response pathways, as suggested by gene ontology analyses .
