FOXD4L1 Antibody

Definition and Molecular Target

FOXD4L1 Antibody (Catalog #A15665-1) is manufactured by Boster Bio and reacts with human and mouse FOXD4L1 proteins . The antibody binds to an 18-amino acid synthetic peptide near the carboxy terminus of human FOXD4L1, ensuring specificity for this transcription factor. FOXD4L1 itself is a member of the FOX family, characterized by its forkhead DNA-binding domain, and functions as both a transcriptional activator and repressor in neural ectoderm formation .

Mechanism of Action

The antibody binds to FOXD4L1 via epitope recognition, enabling detection of the protein in diverse assays. Its specificity is validated through blocking peptide assays, where pre-incubation with the immunogen abolishes signal in Western blot (Fig. A and B in ). This ensures its utility in studying FOXD4L1’s role in:

• Neuroectoderm formation: FOXD4L1 represses neural inhibitors (e.g., ventx1.1) and activates neural genes (e.g., otx2, krox20) .

• BMP signaling modulation: FOXD4L1 inhibits Smad1 nuclear localization and BMP4-target gene expression .

Western Blot

• Recommended dilution: 1 μg/mL.

• Sample type: Tested in mouse lysates (e.g., A-20 cells) .

• Use case: Quantifying FOXD4L1 expression during neural plate development .

Immunocytochemistry

• Recommended dilution: 10 μg/mL.

• Sample type: Human MCF7 cells .

• Use case: Localizing FOXD4L1 in neural progenitor cells .

ELISA

• Sensitivity: Detects FOXD4L1 in low-abundance samples .

Validation Data

Research Context

FOXD4L1’s dual role in transcriptional regulation (activation/repression) is critical for neural ectoderm specification . Studies utilizing this antibody could investigate:

• Neuroectoderm vs. non-neural ectoderm fate decisions .

• Interactions with co-factors like Grg4 or Smad1 .

• Cancer biology, as FOXD4L1 is implicated in oncogenesis .