FOXJ3 Antibody

Cancer Biology

FOXJ3 is overexpressed in breast cancer tissues and cell lines, where it promotes cell proliferation, migration, and invasion by modulating Snail expression, a key driver of epithelial-mesenchymal transition . The FOXJ3 antibody enables detection of FOXJ3 protein levels in cancer models, aiding mechanistic studies and therapeutic target validation .

Autoimmune Diseases

FOXJ3 polymorphisms (e.g., rs585320) are associated with rheumatoid arthritis (RA), particularly in anti-CCP-positive patients . The antibody facilitates genotype-phenotype correlation studies by quantifying FOXJ3 expression in immune cells or patient-derived samples .

Muscle Biology

FOXJ3 regulates skeletal muscle fiber identity and regeneration by activating Mef2c, a transcription factor critical for muscle development . Knockdown or mutation of FOXJ3 disrupts myoblast proliferation and muscle contractility, insights enabled by FOXJ3 antibody-based assays .

Key Research Findings Using FOXJ3 Antibody

Protocols and Best Practices

• Western Blotting: Use 1:500–1:3000 dilution in 5% non-fat milk; detect bands at ~69 kDa .

• Sample Preparation: Lysates from HEK-293 cells or human liver tissue serve as positive controls .

• Troubleshooting: Optimize antibody titration for low-abundance targets; avoid repeated freeze-thaw cycles .

Future Directions

The FOXJ3 antibody remains pivotal for exploring FOXJ3’s role in transcriptional regulation, disease pathogenesis, and therapeutic targeting. Ongoing studies are investigating its utility in single-cell sequencing, chromatin immunoprecipitation (ChIP), and biomarker discovery.