FOXO6 Antibody, FITC conjugated
Description
Overview of FOXO6 Antibodies
FOXO6 (forkhead box protein O6) antibodies are tools used to detect this transcription factor involved in cellular metabolism and muscle differentiation. While no FITC-conjugated versions appear in the provided sources, standard FOXO6 antibodies exhibit these characteristics:
Typical FITC Conjugation in Antibodies
FITC (fluorescein isothiocyanate) conjugation enables direct fluorescence-based detection. Though not explicitly described in the search results, FITC-conjugated antibodies generally follow these protocols:
Common Applications
| Application | Usage |
|---|---|
| Immunofluorescence | Direct visualization of FOXO6 in fixed cells/tissues |
| Flow Cytometry | Quantifying FOXO6 expression in cell populations |
| ELISA | Antigen detection without secondary antibodies |
Key Research Findings on FOXO6
Despite the absence of FITC-specific data, studies highlight FOXO6’s biological roles:
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Muscle Atrophy: FOXO6 knockdown in C2C12 cells reduces myotube differentiation and increases atrophy markers (atrogin1, MURF1) .
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Molecular Weight Variability: Discrepancies in observed molecular weights (51–80 kDa) suggest post-translational modifications or isoform-specific detection .
Limitations and Recommendations
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Conjugate Validation: FITC conjugation requires empirical optimization for each application (e.g., WB vs. IF).
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Species Compatibility: Confirm cross-reactivity with target species (e.g., rat, human) using validation data.
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Phosphorylation States: Phospho-specific antibodies (e.g., ab154832 ) may require distinct validation steps.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
