At4g36750 Antibody

Definition and Target Protein

The At4g36750 Antibody is a polyclonal or monoclonal antibody raised against the protein encoded by the At4g36750 gene. This gene encodes a quinone reductase, a class of enzymes involved in redox reactions and detoxification pathways . The antibody is used to detect and quantify this protein in experimental systems, enabling researchers to study its localization, function, and interactions.

Biological Role of the Target Protein

The At4g36750-encoded protein is associated with detergent-resistant membranes (DRMs) in Arabidopsis, which are lipid-enriched microdomains involved in cellular signaling and trafficking . DRMs often harbor proteins critical for membrane organization, stress responses, and pathogen defense. The quinone reductase activity of this protein suggests a role in modulating redox states or detoxifying reactive oxygen species (ROS), which are common in plant stress responses.

Research Applications

The At4g36750 Antibody is primarily used in:

• Protein Localization: Identifying the subcellular distribution of the target protein (e.g., plasma membrane, endoplasmic reticulum).

• Functional Studies: Investigating its role in redox regulation, stress responses, or membrane dynamics.

• Phosphoproteomics: Analyzing post-translational modifications (PTMs) in response to stimuli like auxin .

Case Study: DRM-Associated Proteins

In a study on Arabidopsis DRMs, the At4g36750 protein was identified alongside other DRM-enriched proteins, including GPI-anchored arabinogalactan proteins (AGPs) and V-type ATPase subunits . This suggests a potential role in membrane organization or signaling pathways regulated by DRMs.

Key Observations

1. DRM Enrichment: The At4g36750 protein is enriched in DRMs, indicating its association with membrane microdomains .

2. Functional Context: Quinone reductases like At4g36750 may protect cells from oxidative damage by reducing quinones (e.g., ubiquinones) or detoxifying harmful metabolites.

3. Auxin-Induced Responses: While not directly studied, auxin-triggered phosphoproteomic changes in Arabidopsis roots highlight the broader relevance of studying stress-responsive proteins .

Limitations and Gaps

• Functional Specificity: Limited data exist on the exact biochemical activity or binding partners of At4g36750.

• Structural Insights: No crystallographic or cryo-EM structures are available for this protein, hindering mechanistic understanding.

• Cross-Species Utility: The antibody’s specificity for Arabidopsis limits its application to other model organisms.

Comparative Analysis with Related Proteins

Source: Analysis of DRM-associated proteins in Arabidopsis

Future Directions

1. Mechanistic Studies: Investigate the role of At4g36750 in ROS detoxification or redox signaling.

2. Interactome Mapping: Identify binding partners using co-immunoprecipitation (Co-IP) with the antibody.

3. Transgenic Models: Engineer At4g36750-knockout plants to assess phenotypic impacts under stress.