At5g58800 Antibody
Description
What Is AT5G58800?
AT5G58800 encodes a quinone reductase, an enzyme involved in redox reactions, electron transport, and detoxification pathways. In plants, quinone reductases may participate in stress responses, such as oxidative stress mitigation or hormone signaling .
| Parameter | Detail |
|---|---|
| Gene ID | AT5G58800 |
| Protein Family | Quinone reductase (NAD(P)H dehydrogenase) |
| EC Number | 1.6.5.2 |
| Function | Catalyzes electron transfer; potential role in stress response pathways |
Hypothetical Role of the At5g58800 Antibody
Antibodies targeting AT5G58800 would serve as tools to study:
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Protein localization: Immunohistochemistry to map quinone reductase distribution in plant tissues.
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Functional interactions: Co-immunoprecipitation to identify protein complexes.
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Expression dynamics: Western blot analysis to quantify enzyme levels under stress conditions (e.g., drought, pathogens).
Antibody Development Strategies
Monoclonal antibodies (mAbs) or polyclonal antibodies could be generated using standard protocols:
3.1. Antigen Preparation
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Recombinant protein production: AT5G58800 cDNA cloned into bacterial systems (e.g., E. coli) for expression and purification .
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Peptide synthesis: Short epitope sequences (e.g., 15–20 amino acids) from AT5G58800 used for immunization.
3.2. Immunization and Screening
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Hybridoma technology: Mice immunized with antigen, B cells fused with myeloma cells to generate hybridomas .
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Recombinant antibody platforms: Phage display or yeast display systems to select high-affinity binders .
3.3. Validation Methods
4.1. Antibody Specificity
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Cross-reactivity: Quinone reductases share conserved motifs; antibodies must distinguish AT5G58800 from homologs.
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Validation gaps: Limited use of knockout (KO) controls in plant studies compared to mammalian systems .
4.2. Functional Relevance
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Redox regulation: AT5G58800’s role in electron transport chains may overlap with other reductases (e.g., cytochrome P450s).
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Stress adaptation: Antibodies could help dissect its contribution to reactive oxygen species (ROS) scavenging.
Comparative Analysis of Antibody Types
| Feature | Monoclonal Antibody | Polyclonal Antibody |
|---|---|---|
| Specificity | High (single epitope) | Moderate (multiple epitopes) |
| Production Time | Months (hybridoma screening) | Weeks (serum harvest) |
| Use Case | Precise localization studies | Broad expression profiling |
Research Gaps and Future Directions
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Lack of existing data: No published studies on AT5G58800-specific antibodies.
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Therapeutic potential: Unlikely for plant-targeted antibodies, but may inform agricultural stress tolerance strategies.
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Collaborative efforts: Partnerships between plant biologists and antibody engineers could advance reagent development .
