FRO2 Antibody
Shipped with Ice Packs 
In Stock 
Description
Protein Complex Formation
FRO2 interacts with IRT1 and AHA2 in root epidermal cells to form a high-affinity iron uptake complex . Co-immunoprecipitation (Co-IP) experiments confirmed:
-
Interaction partners:
Protein Role Method IRT1 Fe²⁺ transporter Co-IP with mCitrine AHA2 Proton-ATPase (pH regulation) Split-Ub assay
This complex enhances iron solubility and uptake efficiency under iron-deficient conditions .
Posttranscriptional Regulation
-
Iron-dependent expression: FRO2 mRNA accumulates under iron starvation but requires low iron for enzymatic activity .
-
Tissue specificity: Expressed in root epidermal cells and anther filaments, critical for iron delivery to pollen grains .
Functional Insights from Overexpression Studies
Transgenic 35S-FRO2 plants show:
-
Enhanced iron uptake: Improved growth on low-iron media due to elevated Fe³⁺ reductase activity .
-
Copper reductase activity: FRO2 reduces Cu²⁺ to Cu⁺, though this role remains secondary to iron uptake .
Technical Considerations
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
FRO2 antibody; FRD1 antibody; At1g01580 antibody; F22L4.12Ferric reduction oxidase 2 antibody; AtFRO2 antibody; EC 1.16.1.7 antibody; Ferric-chelate reductase 2 antibody; Protein FERRIC CHELATE REDUCTASE DEFECTIVE 1 antibody
Target Names
FRO2
Uniprot No.
Target Background
Function
FRO2 is a flavocytochrome that facilitates electron transfer across the plasma membrane. It reduces ferric iron chelates to form soluble ferrous iron in the rhizosphere, contributing to iron delivery to developing pollen grains. Additionally, FRO2 functions as a copper-chelate reductase. It plays a role in glycine betaine-mediated chilling tolerance and the regulation of reactive oxygen species accumulation.
Gene References Into Functions
- Induction of FRO2 gene expression serves as a reliable indicator of iron deficiency in plant roots. PMID: 21334215
- Nitric oxide (NO) acts downstream of auxin to activate FRO2 in response to iron deficiency in roots. PMID: 20699398
- Research has revealed a novel physiological role for FRO2: inhibiting the accumulation of reactive oxygen species (ROS) during chilling conditions. PMID: 18513375
Database Links
Protein Families
Ferric reductase (FRE) family
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Tissue Specificity
Expressed in the epidermal cells of the roots. High expression in lateral roots and root hairs. Detected in leaves, stems, siliques and in flowers in anthers and styles.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
