FRO8 Antibody

Applications of FRMD8 Antibodies in Research

FRMD8 antibodies are utilized in multiple biochemical and cellular assays:

Notes:

• Antibodies conjugated with fluorophores (e.g., Alexa Fluor 350/555) are frequently used for IF .

• In vivo-grade antibodies are required for functional interference studies .

Role in Inflammatory Signaling

FRMD8 stabilizes iRhoms, which are essential for ADAM17 activity. Studies using FRMD8 antibodies have demonstrated:

• TNF-α Shedding: FRMD8 depletion reduces TNF-α release, highlighting its role in inflammatory responses .

• Wnt Pathway Modulation: FRMD8 negatively regulates Wnt signaling by disrupting AXIN1-LRP6 interactions .

Disease Relevance

• Cancer: FRMD8’s regulation of growth factor shedding (e.g., amphiregulin) implicates it in tumor microenvironments .

• Autoimmune Diseases: Dysregulated FRMD8/iRhom interactions may contribute to aberrant cytokine release .

Antibody Performance

• Abcam ab169933: Validated in WB and IHC-P, with specificity confirmed against human FRMD8 .

• Bioss Inc. Polyclonal: Broad applicability across WB, ELISA, and IF, though requires optimization for each assay .

Table 2: Research Applications and Outcomes