FRS7 Antibody
Description
FRS7 Protein Overview
FRS7 (FAR1 RELATED SEQUENCE 7) is a transcription factor in Arabidopsis that partners with FRS12 to regulate photoperiodic flowering and diurnal growth . It integrates into a nuclear repressor complex with histone-like proteins (e.g., HON4) and chromatin modifiers to inhibit gene expression linked to flowering and growth pathways .
Research Tools for Studying FRS7
While no commercial FRS7-specific antibody is described in the provided sources, studies on FRS7 use advanced molecular techniques:
Key Methodologies
Role in Plant Development
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Photoperiodic Flowering: FRS7-FRS12 represses genes downstream of GIGANTEA (GI) and CONSTANS (CO), delaying flowering under non-inductive photoperiods .
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Diurnal Growth Regulation: The complex inhibits growth-promoting genes (e.g., PIF4 targets) during daylight hours .
Protein Interaction Network
| Interactor | Function | Binding Condition |
|---|---|---|
| FRS12 | Core repressor partner | Constitutive in cD/LD |
| HON4 | Chromatin compaction | Nighttime (ZT20) |
| AHL9/AHL14 | Chromatin remodeling | Daytime (ZT4) |
Antibody-Based Detection in FRS7 Studies
Though no FRS7-specific antibody is explicitly mentioned, studies use epitope tags (e.g., HA, GFP) for detection:
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Pro35S:FRS7-HA: HA-tagged FRS7 enabled immunoprecipitation and western blotting .
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TAP-tagged FRS12: Identified FRS7 as a binding partner via mass spectrometry .
Comparative Analysis with FZD7 Antibodies
The second source discusses anti-FZD7 antibodies (targeting Frizzled-7 in cancer), which are unrelated to FRS7 but illustrate antibody development workflows :
| Parameter | Anti-FZD7 scFv (Cancer Research) | FRS7 Detection (Plant Studies) |
|---|---|---|
| Target | Frizzled-7 receptor in breast cancer | Epitope-tagged FRS7 in Arabidopsis |
| Application | Inhibits Wnt/β-catenin signaling | Represses flowering/growth pathways |
| Assays | Flow cytometry, MTT, transwell assays | TAP-MS, ChIP-Seq, BiFC |
Research Gaps and Opportunities
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No vertebrate or human homolog of FRS7 has been reported, limiting antibody development outside plant models.
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Epitope tagging remains the primary method for studying FRS7 due to the lack of species-specific antibodies.
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Function: The FRS7 protein is a putative transcription activator implicated in the regulation of plant development in response to light. Further research suggests a regulatory role within a larger context:
Supporting Evidence:
- The FRS7-FRS12 transcriptional repressor complex influences flowering time and plant growth in Arabidopsis thaliana. PMID: 28492275
Q&A
Here’s a structured FAQ for researchers investigating FRS7 antibodies, aligned with academic research rigor and methodological depth:
Advanced Research Questions
How do I resolve contradictory FRS7 expression data between RNA-seq and Western blot?
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Troubleshooting Framework:
What orthogonal methods confirm FRS7 antibody specificity in single-cell RNA-seq (scRNA-seq) studies?
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Integrated Approach:
How to design a longitudinal study assessing FRS7 as a biomarker in therapy resistance?
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Protocol:
Methodological Tables
Table 1: FRS7 Antibody Validation Parameters
| Parameter | Recommended Method | Acceptance Criteria |
|---|---|---|
| Specificity | siRNA knockdown + Western | ≥80% signal reduction |
| Sensitivity | Serial dilution ELISA | LOD ≤0.5 ng/mL |
| Inter-Assay CV | Triplicate runs (3 days) | CV <15% |
Table 2: Common Pitfalls in FRS7 Studies
| Issue | Solution |
|---|---|
| Non-specific bands | Pre-absorb antibody with blocking peptide |
| Low signal in IHC | Optimize HIER (heat-induced retrieval) |
