FTSH5 Antibody
Description
Introduction to FTSH5 Antibody
The FTSH5 Antibody is a specialized immunological reagent targeting ATP-dependent zinc metalloprotease FTSH5 (VAR1), a critical enzyme in chloroplast thylakoid membranes. This antibody facilitates studies on protein quality control, chloroplast development, and stress responses in plants .
Key Attributes:
FTSH5 is one of 12 FtsH proteases in Arabidopsis, with nine localized to chloroplasts. FTSH1 and FTSH5 are functionally interchangeable in thylakoid membranes .
Biological Context of FTSH5 in Chloroplast Function
FTSH5 plays essential roles in:
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Protein Quality Control: Degrades photo-damaged D1 protein in Photosystem II (PSII) to maintain photosynthesis .
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Stress Responses: Collaborates with zinc-finger transmembrane proteins like FIP to mediate abiotic stress tolerance .
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Photosystem I (PSI) Biosynthesis: Facilitates translation efficiency of PSI core proteins (e.g., PsaA/B) .
Cross-Reactive Species:
The antibody recognizes FTSH5 homologs in 20+ plant species, including:
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Zea mays (maize), Oryza sativa (rice), Glycine max (soybean)
Specificity Validation:
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Homology: 100% sequence match with FTSH1 (AT1G50250), enabling cross-reactivity studies .
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Localization: Confirmed thylakoid membrane localization via chloroplast import assays .
Research Applications in Protein Interaction Studies
The FTSH5 Antibody has been utilized to:
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Identify interacting partners (e.g., FIP) via yeast two-hybrid and pull-down assays .
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Study membrane integration mechanisms using inhibitors (e.g., sodium azide for Sec pathway inhibition) .
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Analyze PSI assembly defects in Arabidopsis mutants (var1, var2) .
Comparative Analysis of FTSH5 and FTSH2 Integration Mechanisms
Both require stromal processing peptidase and thylakoid signal peptidase for maturation .
Role in Photosystem I Biosynthesis
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The AtJ8 protein, which is primarily expressed in darkness, undergoes rapid turnover. However, it is partially stabilized by FTSH proteases. The var1 mutant, lacking the FTSH5 protease, exhibits a near absence of AtJ8. PMID: 21592617
- The Sec pathway is responsible for integrating FtsH5 into thylakoids. PMID: 21214651
