FTSH6 Antibody

Definition and Overview of FtsH6 Antibody

FtsH6 Antibody is a polyclonal rabbit antibody designed to target the ATP-dependent zinc metalloprotease FtsH6 in chloroplasts. This antibody is primarily used to study the protein’s role in plastid function, heat stress responses, and protein degradation pathways. FtsH6 is a nuclear-encoded enzyme localized to thylakoid membranes, where it regulates proteins like HSP21 during heat stress recovery .

Specificity and Cross-Reactivity

The FtsH6 antibody exhibits distinct cross-reactivity profiles depending on the specific product variant:

Note: Cross-reactivity with FTSH2 and FTSH8 in some species may require validation in specific experimental contexts .

Role in Heat Stress and Thermomemory Studies

FtsH6 antibodies have been instrumental in elucidating the protein’s function in heat stress recovery. Key findings include:

1. HSP21 Degradation: FtsH6 regulates HSP21 turnover during the thermomemory phase. Immunoblotting with FtsH6 antibodies revealed that ftsh6 mutants retain higher HSP21 levels, enhancing heat stress memory .

2. Autophagy Synergy: Combined inhibition of FtsH6 and autophagy pathways (e.g., atg18a) leads to greater HSP21 accumulation, underscoring FtsH6’s role in additive protein degradation .

3. Transcriptional Regulation: HSFA2 transcription factor binds to the FtsH6 promoter, modulating its expression during heat stress. Antibody-based assays confirmed FtsH6 induction in primed plants .

Experimental Validation

• Immunoblotting: Used to quantify FtsH6 protein levels after cycloheximide (CHX) treatment, demonstrating reduced FtsH6 synthesis in CHX-treated plants .

• ChIP Experiments: While not explicitly detailed in antibody studies, FtsH6’s promoter regulation by HSFA2 suggests potential applications in epigenetic research .