Customize FTSH7 Antibody

Description

Biological Context of FTSH7

FTSH7 (A. thaliana gene AT3G47060) is a chloroplast-localized protease with dual roles:

Property	Detail
Gene Identifier	AT3G47060 (TAIR)
Protein Name	FTSH protease 7
Localization	Chloroplast thylakoid and envelope membranes
Function	- ABA-dependent dehydration response - Protein quality control and refolding - Thermotolerance regulation (indirectly via FTSH homologs)

FTSH7 forms heterohexameric complexes with other FTSH subunits (e.g., FTSH1, FTSH2) to maintain chloroplast integrity under stress . Its protease and chaperone activities are essential for degrading misfolded proteins and reassembling functional complexes like the DrrAB transporter in bacteria .

Key Features

Host Species: Rabbit
Immunogen: KLH-conjugated peptide derived from FTSH7 sequences
Reactivity:
- Plants: A. thaliana, Hordeum vulgare, Zea mays
- Algae: Bryopsis hypnoides, Synechocystis sp.
- Diatoms: Thalassiosira pseudonana
Molecular Weight: ~87.8 kDa (chloroplastic isoform)
Applications: Western Blot, ELISA, Immunohistochemistry

Supplier Data

Supplier	Catalog Number	Status	Target Specificity
PhytoAB	AT3G47060	Out of stock	ATP-dependent zinc metalloprotease FTSH7
Agrisera	AS11 1789	Available	Pan-FTSH (cross-reactive with 11 isoforms)

Protein Degradation Studies

FTSH7 antibodies have been used to investigate:

Thylakoid Membrane Dynamics: FTSH7 destabilizes unassembled subunits of photosynthetic complexes (e.g., cytochrome b₆f) during light stress .
Chaperone Activity: FTSH proteases refold misassembled membrane proteins, as shown in E. coli DrrAB complex rescue experiments .

Stress Response Mechanisms

Dehydration and ABA Signaling: FTSH7 is upregulated during ABA-mediated drought responses, with knockout mutants showing impaired stress recovery .
Thermotolerance: While FTSH11 directly regulates heat tolerance, FTSH7’s interaction with CPN60 chaperonins suggests overlapping roles in protein stabilization .

Challenges and Limitations

Cross-Reactivity: Anti-FTSH7 antibodies may detect other FTSH isoforms (e.g., FTSH1, FTSH5) due to sequence homology .
Localization Conflicts: Early studies suggested dual mitochondrial/chloroplast localization, but recent work confirms chloroplast-specific activity .
Phenotypic Redundancy: Single ftsH7 mutants often lack visible phenotypes, necessitating combinatorial protease knockouts for functional studies .

Future Directions

Substrate Identification: Use immunoprecipitation-MS to identify FTSH7-specific targets like TIC40 or PAPST2 .
Structural Studies: Cryo-EM of FTSH7 complexes could reveal refolding mechanisms .
Agricultural Engineering: Modulating FTSH7 expression may enhance crop resilience to drought and heat .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

FTSH7 antibody; At3g47060 antibody; F13I12.110 antibody; ATP-dependent zinc metalloprotease FTSH 7 antibody; chloroplastic antibody; AtFTSH7 antibody; EC 3.4.24.- antibody

Target Names

FTSH7

Uniprot No.

Q9SD67

Target Background

Function

This antibody targets FTSH7, a protein that likely functions as an ATP-dependent zinc metallopeptidase.

Database Links

KEGG: ath:AT3G47060

STRING: 3702.AT3G47060.1

UniGene: At.43767

Protein Families

AAA ATPase family; Peptidase M41 family

Subcellular Location

Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein; Stromal side.

Q&A

Here’s a structured FAQ collection for researchers working with FTSH7 antibody, designed to address methodological and analytical challenges in academic research:

Advanced Research Questions

How to resolve contradictions in FTSH7 interaction data across studies?
- Issue: Some studies report FTSH7 interactions with FtsHi1/4/5 , while others find no association .
- Methodological adjustments:
  - Compare extraction buffers: Use mild detergents (e.g., 1% digitonin) to preserve weak interactions .
  - Validate with orthogonal methods (e.g., split-ubiquitin yeast two-hybrid vs. Co-IP/MS) .
  - Account for tissue-specific expression by testing multiple plant organs .
What controls are critical for interpreting FTSH7 immunoprecipitation data?
Include:
- Negative controls:
  - Wild-type samples without epitope tags .
  - Beads without antibody .
- Cross-species controls: Test antibody reactivity in non-model species (e.g., Zea mays) using peptide pre-absorption .

Table 1: FTSH7 Interaction Partners Identified via Co-IP/MS

Interactor	Function	Study Context	Confirmation Method
FtsHi1	ATPase subunit	Chloroplast envelope	Co-IP, genetic mutants
pdNAD-MDH	Malate dehydrogenase	Metabolic regulation	Affinity purification
Ribosomal proteins	Translation machinery	Stress recovery	MS/MS
No detection	FTSH11, FTSH9	Envelope proteomics	Knockout validation

Table 2: Antibody Performance Across Species

Species	Reactivity	Optimal Dilution	Notes
Arabidopsis thaliana	Strong	1:1,000	Validated in ftsh7 mutants
Zea mays	Moderate	1:500	Requires cross-adsorption
Synechocystis sp.	Weak	1:200	Use with enhanced chemiluminescence

Methodological Recommendations

How to optimize FTSH7 detection in low-abundance samples?
- Signal amplification: Use tyramide-based amplification for immunofluorescence .
- Fractionation: Enrich chloroplast membrane fractions via sucrose density gradients .
- Quantification: Pair immunoblotting with stable isotope labeling (SILAC) for absolute quantification .
What bioinformatics tools complement FTSH7 antibody studies?
- Localization prediction: ChloroP, TargetP for chloroplast transit peptides .
- Interaction networks: STRING database to map FTSH7-associated pathways .

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FTSH7 Antibody