FTSZ2-1 Antibody
Description
Introduction to FtsZ2-1 Antibody
The FtsZ2-1 Antibody is a polyclonal rabbit antibody specifically designed to detect the FtsZ2-1 protein, a homolog of bacterial FtsZ critical for chloroplast division in plants. This antibody targets the conserved regions of FtsZ2-1, enabling precise localization and quantification in experimental systems. Its primary applications include Western blotting, immunoprecipitation, and immunofluorescence, with validation across diverse plant species .
Species Reactivity
The FtsZ2-1 Antibody demonstrates high specificity for Arabidopsis thaliana and Hordeum vulgare, with predicted reactivity in other plant species, including Glycine max, Ricinus communis, and Populus trichocarpa .
| Species | Reactivity | Source |
|---|---|---|
| Arabidopsis thaliana | Confirmed | |
| Hordeum vulgare | Confirmed | |
| Glycine max | Predicted | |
| Populus trichocarpa | Predicted |
Cross-Reactivity and Validation
The antibody does not cross-react with bacterial FtsZ or tubulin-like proteins . Competitive immunoblot assays confirm its specificity, as pre-incubation with recombinant FtsZ2-1 blocks signal detection, while FtsZ1-1 does not .
Western Blotting
The FtsZ2-1 Antibody is optimized for Western blotting at a dilution of 1:2000, detecting a band of ~50.7 kDa (expected apparent molecular weight) . Example protocols include:
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Sample Preparation: Separation of recombinant FtsZ2-1 (~10 kDa) or plant extracts on 4–12% LDS-PAGE.
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Detection: Blocking with 2% low-fat milk, probing with the antibody, and signal development using HRP-conjugated secondary antibodies .
Co-Immunoprecipitation (Co-IP)
Used in Physcomitrella to confirm in vivo interactions between FtsZ2-1 and FtsZ1 isoforms. GFP-tagged FtsZ2-1 was immunoprecipitated, and interacting partners were identified via mass spectrometry .
Immunofluorescence
Dual-labeling studies in Arabidopsis reveal colocalization of FtsZ2-1 and FtsZ1-1 at mid-chloroplast rings, critical for plastid division .
Functional Redundancy of FtsZ2 Isoforms
Studies in Arabidopsis demonstrate that FtsZ2-1 and FtsZ2-2 are functionally redundant for chloroplast division, while FtsZ1 is non-redundant. Overexpression of either FtsZ2 isoform rescues division defects in null mutants, but replacement of FtsZ1 with FtsZ2 fails to restore normal plastid numbers .
Synergistic Interactions with FtsZ1
In Physcomitrella, FtsZ2-1 interacts with FtsZ1-2 in vitro and in vivo, enhancing GTPase activity and polymerization rates. This synergistic interaction is distinct from bacterial FtsZ dynamics .
Role in Chloroplast Division
FtsZ2-1 is essential for plastid division in higher plants. Overexpression disrupts division, while antisense suppression reduces plastid numbers. The antibody has been pivotal in tracking FtsZ2-1 levels during these experiments .
Evolutionary Conservation
FtsZ2-1 shares structural homology with bacterial FtsZ but lacks motor proteins. Its function in plants suggests a conserved mechanism of cytoskeletal ring formation for organelle division .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
This antibody targets FtsZ2-1, a protein exhibiting GTPase activity. It is a critical component of the plastid division machinery, forming a contractile ring at the division site. FtsZ2-1's role in plastid division is dose-dependent.
FtsZ2-1 Function and Regulation: The following research highlights key aspects of FtsZ2-1's function and regulation within the context of plastid division:
- ARC3 Regulation: Chloroplast division protein ARC3 influences FtsZ2 by preventing filament bundling and enhancing its GTPase activity. (PMID: 29138260)
- Developmental Regulation: FtsZ2-2's effects are more pronounced in shoot apices than mature leaves, suggesting developmental regulation of plastid proliferation. (PMID: 29920253)
- FtsZ1/FtsZ2 Interaction: FtsZ1 and FtsZ2 can heteropolymerize into a contractile ring. Co-assembly imparts polarity to polymerization. (PMID: 27322658)
- PARC6 Inhibition: PARC6 negatively regulates FtsZ assembly. (PMID: 26527658)
- ARC3 and FtsZ Turnover: In plants lacking ARC3 (a key negative regulator), FtsZ1/FtsZ2 turnover is significantly slower. (PMID: 25731613)
- Spatial Regulation of ARC3: ARC3 directly inhibits Z-ring assembly via interaction with FtsZ2. MinD1 and MinE1 spatially regulate ARC3 activity. (PMID: 23715471)
- FtsZ Filament Assembly: FtsZ1 and FtsZ2-1 self-assemble into distinct filament types (type-I and type-II) with differing assembly intermediates. (PMID: 21781955)
- FtsZ2-1 and FtsZ2-2 Roles: FtsZ2-1 is essential for chloroplast division, while FtsZ2-2 contributes to chloroplast morphology and internal organization. (PMID: 21439281)
- GTPase Activity Differences: FtsZ1-1 and FtsZ2-1 exhibit distinct GTPase activities. (PMID: 19925792)
- FtsZ Complex Composition: Studies have quantified the in vivo relationship between FtsZ1 and FtsZ2 and identified ARC6 and ARC3 within a native FtsZ complex. (PMID: 18284374)
