FUT12 Antibody

Introduction to FUT12 Antibody

FUT12 Antibody (also recognized as POFUT1 Antibody) is a research-grade polyclonal antibody targeting FUT12 (Peptide-O-fucosyltransferase 1), a critical enzyme in the glycosylation of epidermal growth factor (EGF)-like protein domains (N-linked glycosylation). This antibody enables the detection and study of FUT12 in various biological contexts, including its role in Notch signaling, protein folding, and diseases linked to aberrant glycosylation .

Table 1: Biochemical Properties of FUT12

Functional Insights:

• Notch Signaling: FUT12-dependent O-fucosylation is required for ligand binding to Notch receptors, regulating cell fate decisions and development .

• Essential in Mammals: Genetic knockout of FUT12 in mice is embryonically lethal, highlighting its critical role in development .

Table 2: Observed Band Sizes in Western Blotting

Key Applications:

• Protein Glycosylation Studies: Used to analyze FUT12 expression in cancers, developmental disorders, and immune-related diseases .

• Cancer Research: FUT12 activity is linked to tumor progression and metastasis, with antibodies aiding in biomarker discovery .

Table 3: Species Reactivity and Predictions

Specificity Notes:

• Immunogen: Peptides derived from FUT12’s AA 300–380 region ensure specificity .

• Controls: Pre-absorption with immunizing peptides eliminates non-specific signals .

Table 4: Therapeutic Relevance of FUT12-Related Pathways

Emerging Applications:

• Targeted Therapies: Inhibiting FUT12 could disrupt Notch signaling in cancers, though no clinical trials are currently reported.

• Biomarker Development: FUT12 expression levels may predict disease progression or treatment response .