FZR2 Antibody

Research Applications and Key Findings

FXR2 Antibody has been employed in diverse experimental contexts:

Molecular Mechanisms

• RNA Regulation: FXR2 interacts with FMR1 and FXR1 to regulate mRNA translation and stability, particularly in neurogenesis .

• Viral Infection: FXR2 binds Sindbis virus non-structural protein 3, inhibiting host stress granule formation and promoting viral replication .

• PTMs: FXR2 undergoes extensive phosphorylation (e.g., S6, S21, Y26) and ubiquitination (e.g., K205, K256), influencing its function .

Experimental Uses

Research Publications and Functional Insights

Recent studies highlight FXR2’s role in:

1. Neurogenesis: FXR2 regulates NOG mRNA translation, modulating hippocampal neurogenesis .

2. Viral Pathogenesis: FXR2-nsp3 interactions facilitate viral RNA replication in Sindbis virus infection .

3. Alzheimer’s Disease: FXR2 may influence BACE1 translation via ARL6IP1-mediated pathways .

Protocols and Optimization

Optimal antibody performance requires:

• Western Blot: Use denatured protein samples; validate with peptide ELISA .

• IHC: Antigen retrieval with TE buffer pH 9.0 or citrate buffer pH 6.0 .

• IP: Use 0.5–4.0 µg antibody per 1–3 mg lysate .

Protocols for WB, IHC, IF, and IP are available for Proteintech’s 12552-1-AP antibody .

References and RRIDs

Citations should include:

• DF3195: Affinity Biosciences Cat# DF3195, RRID:AB_2835554 .

• 12552-1-AP: Proteintech Cat# 12552-1-AP, RRID:AB_2110720 .