GA2OX4 Antibody
Description
Functional Role of GA2OX4
GA2OX4 belongs to the GA2ox class of enzymes that convert bioactive GAs (e.g., GA₁, GA₄) into inactive forms (e.g., GA₈, GA₃₄) via 2β-hydroxylation. This activity limits GA levels, influencing processes like stem elongation, seed germination, and stress adaptation .
Substrate Specificity
| Substrate | Product | Enzyme Activity |
|---|---|---|
| GA₉ | GA₅₁ | C₁₉-GA oxidation |
| GA₄ | GA₃₄ | Bioactive GA inactivation |
| GA₁ | GA₈ | Developmental regulation |
Genetic and Molecular Insights
-
Gene Structure: In Arabidopsis thaliana, GA2OX4 (AT1G47990) is part of a five-member GA2ox family (classes I and II) with distinct tissue-specific expression .
-
Regulatory Mechanisms:
Protein Interactions and Degradation
-
Ubiquitination: GmGA2ox-like (a soybean homolog) interacts with E3 ligase GmILPA1, which targets lysine 394 for ubiquitination, promoting proteasomal degradation under UV-B stress .
-
Enzymatic Assays: Recombinant GA2OX4 converts GA₄ to GA₃₄ in vitro (confirmed via HPLC and GC-MS) .
Phenotypic Impacts
-
Dwarfism: Overexpression of GA2OX4 or its homologs reduces bioactive GA levels, leading to dwarf phenotypes in Arabidopsis and soybean .
-
Stress Adaptation: GA2OX4 activity is upregulated under UV-B irradiation, modulating GA homeostasis to enhance stress resilience .
Antibody Applications
While the provided sources do not explicitly describe GA2OX4 antibody validation, standard protocols for plant antibody characterization include:
-
Western Blot: Detects GA2OX4 in protein extracts (e.g., using GFP-tagged constructs) .
-
Immunoprecipitation: Validates interactions with regulatory proteins like GmILPA1 .
-
Tissue Localization: Employed in immunofluorescence to map GA2OX4 expression in roots, stems, and floral tissues .
Research Gaps and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
