GA2OX6 Antibody

Introduction to GA2OX6 and Its Antibody

GA2OX6 (Gibberellin 2-oxidase 6) is a key enzyme in the catabolism of bioactive gibberellins (GAs), plant hormones critical for growth and development. Antibodies targeting GA2OX6 are essential tools for studying its expression, localization, and regulatory roles. These antibodies enable detection via techniques like Western blotting, immunoprecipitation, and immunohistochemistry, facilitating research into GA metabolism and plant developmental biology .

Genetic and Biochemical Characterization of GA2OX6

GA2OX6 belongs to the GA2ox family, which inactivates GAs by 2β-hydroxylation. Key findings include:

• Substrate Specificity: GA2OX6 acts exclusively on C₁₉-GAs (e.g., GA₄, GA₁) and does not target C₂₀-GAs like GA₁₂ or GA₅₃ (Table 1) .

• Phylogenetic Classification:

  • Class I and II GA2ox enzymes target C₁₉-GAs, while Class III targets C₂₀-GAs .

  • In Arabidopsis, GA2OX6 clusters with Class II GA2oxs, distinct from Class I (GA2OX1–3) and Class III enzymes .

Table 1: Substrate Specificity of GA2OX6

Role of GA2OX6 in Plant Development

• Arabidopsis: GA2OX6 is upregulated during somatic embryogenesis and seed development, where it reduces bioactive GA levels to maintain embryonic programs . Knockdown mutants show partial disruption of somatic embryogenesis, suggesting redundancy with other GA2oxs .

• Rice: Overexpression of GA2OX6 mutants (e.g., alanine-substituted variants) leads to semi-dwarfism, increased tillering, and enhanced stress tolerance, highlighting its role in GA homeostasis .

• Peach: GA2OX6 expression is induced by GA₃ treatment, indicating feedback regulation to modulate GA levels dynamically .

Expression Patterns and Regulatory Mechanisms

• Tissue-Specific Expression:

  • High in Arabidopsis roots and siliques, with transient peaks during seed germination .

  • In rice, mRNA accumulates post-vegetative growth, correlating with tiller development .

• Transcriptional Regulation:

  • Directly activated by the MADS-domain protein AGL15 in Arabidopsis, linking GA2OX6 to embryonic gene networks .

  • GA₃ treatment induces rapid transcriptional activation in peach (within 0.5–4 hours) .

Biotechnological Applications

• Crop Improvement:

  • Ectopic expression of GA2OX6 mutants in rice enhances yield (10–30% increase) and abiotic stress tolerance by fine-tuning GA levels .

  • In Jatropha, overexpression reduces bioactive GA content, causing dwarfism and delayed flowering .

• Gene Editing: CRISPR-mediated modulation of GA2OX6 offers potential for optimizing plant architecture in crops .