GA2OX8 Antibody

Target Specificity

The GA2OX8 Antibody specifically recognizes the GA2OX8 enzyme (UniProt ID: O49561), which belongs to the 2-oxoglutarate-dependent dioxygenase (2-ODD) family. Key characteristics include:

• Substrate Specificity: Hydroxylates C20 GAs (e.g., GA12, GA53) but not C19 GAs (e.g., GA1, GA4).

• Tissue Expression: Strong activity in vascular tissues (xylem, phloem) and the root stem cell niche (SCN), particularly the quiescent center (QC) .

• Regulation: Expressed in response to auxin signals, with shoot-derived auxin influencing root elongation zones and vascular development .

Role in Plant Development

GA2OX8 Antibody has been instrumental in elucidating the enzyme’s role in:

• Root Cell Elongation: GA2OX8 deactivates GAs, inhibiting root elongation. Overexpression reduces GA levels, while mutants (e.g., ga2ox heptuple) exhibit elongated roots .

• Vascular Development: Expressed in vascular tissues, GA2OX8 may regulate GA levels to modulate xylem/phloem differentiation .

• Stem Cell Niche Regulation: High GA2OX8 expression in QC correlates with auxin maxima. Mutants show expanded QC and increased columella cell layers, suggesting GA2OX8 controls cell proliferation rates .

Experimental Validation

GA2OX8 Gene (AT4G21200)

Antibody Validation

While specific validation data (e.g., Western blot, immunoprecipitation) are not publicly disclosed, the antibody’s design aligns with established protocols for GA2OX isoforms:

• Recombinant Protein: Produced via yeast expression systems, ensuring proper post-translational modifications .

• Purity: >90% purity, suitable for downstream applications like ELISA .

Limitations

• Cross-Reactivity: Potential off-target binding to homologous GA2OX enzymes (e.g., GA2OX2, GA2OX6) requires validation.

• Tissue Specificity: Requires optimization for detection in diverse plant tissues (e.g., flowers, stems).