GAPCP2 Antibody
Description
Research Findings: Functional Roles of GAPCp2
GAPCp2 is integral to plastidial glycolysis and metabolic regulation. Key insights include:
Immune Response Regulation
GAPDH isoforms, including GAPCp2, negatively regulate plant immune responses:
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Enhanced Disease Resistance: gapcp1gapcp2 knockout (KO) lines showed reduced bacterial titers of Pseudomonas syringae pv. tomato (Pst DC3000) and accelerated hypersensitive response (HR) .
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ROS and Autophagy Modulation: GAPCp2 influences reactive oxygen species (ROS) accumulation and autophagy, critical for pathogen defense .
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Priming Effects: Basal upregulation of pathogenesis-related genes (e.g., PR1) in GAPDH KO lines suggests constitutive immune priming .
Glycolytic and Metabolic Functions
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Non-Phosphorylating Pathway: GAPCp2 participates in chloroplast glycolysis, bypassing ATP production to optimize carbon flux .
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ATP/ADP Dynamics: Loss of plastidial GAPDH (GAPCp1/GAPCp2) alters ATP/ADP ratios, impacting redox balance and glycolytic flux .
Subcellular Localization and Tissue-Specific Expression
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Plastid Localization: Confocal microscopy confirms GAPCp2 presence in leaf epidermal cell plastids, critical for serine synthesis and starch metabolism .
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Tissue Expression: Strong expression in photosynthetic tissues (e.g., leaves) and roots, with minimal activity in non-photosynthetic organs .
Applications of GAPCP2 Antibody
The antibody is widely used in plant biology research:
Comparative Analysis: GAPCp2 vs. Other GAPDH Isoforms
GAPCp2 differs significantly from cytosolic isoforms (GAPC1/GAPC2) and other plastidial forms (GAPCp1):
| Feature | GAPCp2 | GAPC1/GAPC2 | GAPCp1 |
|---|---|---|---|
| Localization | Chloroplast/plastid | Cytosol | Chloroplast/plastid |
| Function | Non-phosphorylating glycolysis, serine synthesis | Phosphorylating glycolysis, ATP production | Similar to GAPCp2 |
| Immune Role | Negative regulator of HR and ROS accumulation | Negative regulator of HR, autophagy | Overlapping with GAPCp2 |
| Expression | High in leaves, roots; low in non-photosynthetic tissues | Ubiquitous, stress-responsive | Similar to GAPCp2 |
Critical Insights from Knockout Studies
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GAPCp1/GAPCp2 Double KO:
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Cytosolic vs. Plastidial KO:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research findings suggest that the primary function of GAPCP2 is to supply 3-PGA for anabolic pathways within the plastids of heterotrophic cells. This research also indicates that plastidial glycolysis may contribute to fatty acid biosynthesis in seeds. PMID: 27984670
- Mutants lacking functional GAPCP1 and GAPCP2 exhibit insensitivity to abscisic acid during growth. PMID: 21068209
- Two isoforms of GAPDH (GAPCp1 and GAPCp2) have been identified in *Arabidopsis thaliana* and are localized within chloroplasts and plastids. PMID: 19675149
