G6PD3 Antibody

G6PD Antibodies: Overview and Function

G6PD antibodies are immunological tools designed to detect or quantify glucose-6-phosphate dehydrogenase (G6PD), a cytosolic enzyme critical for the pentose phosphate pathway. These antibodies are used in research, diagnostics, and therapeutic development to study G6PD’s role in oxidative stress, hemolytic anemia, and cancer biology.

Research Applications of G6PD Antibodies

G6PD antibodies are pivotal in studying G6PD deficiency, oxidative stress, and cancer immunology.

Oxidative Stress and Hemolytic Anemia

G6PD deficiency, caused by mutations in the X-linked G6PD gene, leads to impaired NADPH production, increasing susceptibility to oxidative damage in erythrocytes. Antibodies enable detection of G6PD protein levels in:

• Erythrocytes: Diagnosing G6PD deficiency .

• Lymphocytes: Assessing oxidative stress in immune cells .

Table 1: G6PD Antibody Use in Hemolytic Anemia Studies

Cancer Immunotherapy

G6PD inhibition induces immunogenic cell death (ICD) in tumors, enhancing immunotherapy efficacy. Antibodies are used to:

• Validate G6PD knockdown: Confirm target protein depletion in melanoma and lung cancer models .

• Assess NADPH levels: Monitor oxidative stress in cancer cells treated with G6PD inhibitors .

Table 2: G6PD Antibody Applications in Oncology

G6PD Deficiency Management

Antibodies aid in identifying G6PD-deficient patients at risk for hemolysis triggered by drugs (e.g., primaquine, sulfonamides) or infections .

Small-Molecule Correctors

AG1, a small molecule, stabilizes mutant G6PD (e.g., Canton R459L) and restores enzymatic activity, reducing oxidative stress in erythrocytes and zebrafish models . Antibodies confirm increased G6PD stability post-AG1 treatment.

Autoimmune and Allergic Associations

G6PD-deficient individuals show higher rates of autoimmune disorders (e.g., rheumatoid arthritis, lupus) and allergies (e.g., allergic conjunctivitis). Antibody-based serology studies support these associations .