G6PD6 Antibody
Description
Overview of G6PD Antibodies
G6PD antibodies are immunological tools designed to detect and quantify the G6PD enzyme, a critical catalyst in the pentose phosphate pathway (PPP). This enzyme generates NADPH, essential for redox homeostasis and antioxidant defense systems . Antibodies targeting G6PD enable researchers to study its expression, localization, and dysfunction in diseases ranging from viral infections to autoimmune disorders .
Applications in Research and Diagnostics
G6PD antibodies are widely used in:
Role in Viral Infections
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COVID-19: Postmortem lung biopsies showed elevated G6PD levels in COVID-19 patients (p = 0.001), suggesting NADPH upregulation to counteract oxidative stress .
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Coronavirus Susceptibility: G6PD-deficient cells exhibit increased viral replication due to ROS accumulation and glutathione depletion .
Autoimmune and Inflammatory Diseases
A 14-year cohort study of 7,473 G6PD-deficient individuals revealed significantly elevated risks for:
| Condition | Odds Ratio (95% CI) | p-value |
|---|---|---|
| Systemic Lupus Erythematosus | 4.56 (2.46–8.48) | <0.001 |
| Rheumatoid Arthritis | 2.41 (1.85–3.12) | <0.001 |
| Scleroderma | 6.87 (2.49–20.59) | <0.001 |
Autoantibodies (e.g., anti-Smith, anti-RNP68) were also more prevalent in G6PD-deficient cohorts .
Diagnostic Thresholds
Universal thresholds for G6PD deficiency:
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Severe Deficiency (<30% activity): Sensitivity = 97%, specificity = 99% at 2.8 U/g Hb .
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Moderate Deficiency (70% activity): Higher misclassification rates (OR = 1.81–5.00 for false negatives) .
Mechanistic Insights from Antibody-Based Studies
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Antioxidant Defense: G6PD antibodies confirmed enzyme upregulation during oxidative stress, protecting endothelial cells from TNFα-induced damage .
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T Cell Activation: G6PD inhibition reduces IFN-γ production by 40–60%, highlighting its role in adaptive immunity .
Limitations and Future Directions
Product Specs
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
This antibody targets 6-phosphogluconate dehydrogenase (6PGDH), a key enzyme in the oxidative pentose phosphate pathway. This pathway represents an alternative carbohydrate dissimilation route to glycolysis. The primary function of 6PGDH is to generate reducing power (NADPH) and pentose phosphates, essential precursors for fatty acid and nucleic acid biosynthesis, crucial for membrane synthesis and cell division.
Relevant research highlights the significance of 6PGDH:
- Studies indicate that G6PD6 (encoding 6PGDH) loss alters cellular redox states, increasing plant sensitivity to high salinity stress. PMID: 22885737
- In contrast to the significant reduction observed in most G6PDH isoforms under inorganic phosphate starvation, 6PGDH gene expression reduction was less pronounced. PMID: 19040112
