GALS1 Antibody
Description
GALS1: A β-1,4-Galactan Synthase
GALS1 catalyzes the elongation of β-1,4-galactan chains in pectin, a critical structural polysaccharide in plant cell walls. Its activity is essential for maintaining cell wall integrity and plant growth .
Antibodies in GALS1-Related Research
While no antibodies directly targeting GALS1 are described, LM5 (a monoclonal antibody) is widely used to detect β-1,4-galactan, enabling indirect assessment of GALS1 activity.
GALS1 Overexpression and Knockdown Studies
Overexpression: Ectopic expression of GALS1 in Arabidopsis increases β-1,4-galactan content by ~50%, as shown by LM5 staining and enzymatic assays .
Knockdown: gals1 mutants exhibit reduced galactan, impaired cell wall structure, and altered RG-I sidechain composition .
Biochemical Characterization of GALS1
In vitro assays demonstrate GALS1’s enzymatic specificity:
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Substrate Preference: Linear β-1,4-galacto-oligosaccharides (≥4 units) are required for activity .
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Activity Levels: Optimal with galactopentaose, galactohexaose, or galactoheptaose .
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Inhibition: Branched acceptors reduce activity, confirming linear chain preference .
Functional Implications of GALS1
GALS1’s role extends to pectin remodeling and interactions with other cell wall components:
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RG-I Sidechains: Reduced binding of RU1/RU2 (RG-I backbone) and LM6 (α-1,5-arabinan) antibodies in gals1 mutants .
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Galactan Synthase Network: GALS2 and GALS3 contribute to galactan biosynthesis but are less active than GALS1 .
Comparative Analysis of GALS1 and Related Proteins
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
This antibody targets GALS1, an enzyme involved in the biosynthesis of β-1,4-galactan. In vitro studies demonstrate its ability to transfer galactose residues from UDP-galactose to β-1,4-galactopentaose, specifically forming β-1,4-galactosyl linkages and adding successive β-1,4-galactosyl residues to the acceptor molecule. β-1,4-galactans are prevalent polysaccharides in plant cell walls, serving as side chains of rhamnogalacturonan I, a major pectin component.
References:
- GALS1's ability to specifically form β-1,4-galactosyl linkages and add successive β-1,4-galactosyl residues to the acceptor has been demonstrated. [GALS1] PMID: 23243126
