GAMT2 Antibody

Physiological Roles

GAMT2 is predominantly expressed in developing seeds and siliques, where it modulates GA levels to ensure proper seed maturation and germination. Overexpression of GAMT2 phenocopies GA-deficient mutants, causing dwarfism, delayed flowering, and reduced fertility . Knockout mutants accumulate active GAs, leading to hypersensitivity to GA biosynthesis inhibitors (e.g., ancymidol) .

Antibody Design and Production

While direct data on commercial GAMT2 antibodies is limited, studies on analogous enzymes (e.g., GAMT1) provide insights:

• Antigen Source: Recombinant GAMT2 protein purified from heterologous systems (e.g., E. coli or insect cells).

• Host Species: Typically rabbit, goat, or mouse for polyclonal/monoclonal antibodies.

• Epitope Targeting: Likely regions with conserved functional motifs (e.g., SAM-binding domains).

Validation Methods

Functional Studies

• GA Metabolism: Antibodies enable quantification of GAMT2 protein levels, correlating enzyme activity with GA methylation rates .

• Seed Development: Immunolocalization studies reveal GAMT2’s role in regulating GA inactivation during seed maturation .

Comparative Analysis

Technical Constraints

• Antibody Specificity: Cross-reactivity with GAMT1 or other SABATH family members may occur, necessitating rigorous validation .

• Signal Sensitivity: Low GAMT2 expression in non-seed tissues may require optimized detection protocols (e.g., signal amplification) .

Emerging Opportunities

• CRISPR-Cas9 Editing: Knockout mutants (e.g., SALK_075450) provide negative controls for antibody validation .

• High-Throughput Screens: Antibodies could enable large-scale studies of GA metabolism in crop species.