GASA2 Antibody
Description
Introduction to GAS2 Antibody
The GAS2 Antibody (Growth Arrest-Specific 2 Antibody) is a polyclonal rabbit IgG antibody designed to target the GAS2 protein, a 35 kDa protein encoded by the GAS2 gene (Gene ID: 2620). It is primarily used in research applications such as Western blot (WB), immunohistochemistry (IHC), and ELISA. The antibody is produced via antigen affinity purification using a GAS2 fusion protein immunogen (Ag2540) and demonstrates reactivity with human, mouse, and rat samples .
Target and Reactivity
| Parameter | Details |
|---|---|
| Target | GAS2 protein (GenBank Accession: BC040470; UniProt ID: O43903) |
| Reactivity | Human, mouse, rat |
| Molecular Weight | 35 kDa (calculated: 313 aa) |
| Host/Isotype | Rabbit IgG |
| Purification | Antigen affinity purification |
| Storage | -20°C in PBS with 0.02% sodium azide and 50% glycerol |
Tested Applications and Dilutions
Validated Uses
The GAS2 Antibody has been validated for:
-
WB: Detects GAS2 in human kidney, liver, Jurkat cells, and mouse liver .
-
IHC: Demonstrated staining in human lung cancer tissue with optimized antigen retrieval protocols .
-
ELISA: Confirmed reactivity with GAS2 but lacks detailed assay parameters .
Published Studies
One publication has utilized this antibody in WB experiments, though specific details about the study’s findings are not disclosed in available sources .
Tissue Expression and Validation Data
Data from the Human Protein Atlas indicate:
-
Tissue Expression: Medium consistency between antibody staining and RNA expression in brain tissue.
-
Single-Cell Analysis: Limited data, but GAS2 expression is observed in specific cell types.
-
Validation Methods:
GAS2 Protein Function
The GAS2 protein is associated with cellular processes such as growth arrest and cytoskeletal organization. While its exact role in disease remains under investigation, antibodies targeting GAS2 are critical for studying its expression in cancer (e.g., lung cancer) and normal tissues .
Antibody Specificity
The GAS2 Antibody binds to epitopes on the GAS2 protein, enabling precise detection in diverse experimental models. Its polyclonal nature ensures broader epitope recognition compared to monoclonal antibodies, though this may increase off-target binding risks .
Challenges and Considerations
-
Antigen Retrieval: IHC protocols require optimization (e.g., TE vs. citrate buffer) .
-
Cross-Reactivity: Polyclonal antibodies may bind non-specific epitopes; validation with independent antibodies is recommended .
-
Storage Stability: Long-term storage at -20°C is critical; aliquoting is unnecessary .
