GASA4 Antibody
Description
Developmental Roles
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Meristem Activity: GASA4 is expressed in vegetative, floral, and root meristems, with GA-dependent regulation confirmed in ga1-3 mutants .
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Flowering Control: Overexpression accelerates flowering by modulating FT and LFY expression, while suppression delays it .
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Seed Development: Transcripts peak during silique maturation (5 days post-pollination) and influence seed size/yield .
Stress Responses
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Redox Regulation: AtGASA4 reduces reactive oxygen species (ROS) accumulation, enhancing tolerance to heat and salt stress .
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Germination: Overexpression lines show insensitivity to nitric oxide donors (e.g., SNP) due to redox activity .
Applications of GASA4 Antibody in Research
The antibody has been instrumental in:
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Protein Localization: Immunoblotting confirmed GASA4 presence in flower buds, seedlings, and roots .
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Expression Studies: GUS reporter assays using the GASA4 promoter revealed meristem-specific activity .
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Functional Analysis: Characterizing interactions with VH1/BRL2 kinase for vein development .
Future Directions
Current studies focus on:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The N-terminal signal peptide of GASA4 and GASA6 is cleaved as predicted, and this cleavage is essential for their proper sub-cellular localization. PMID: 26993231
- Overexpression of GASA4 promotes germination and flowering, while inhibiting H2O2 accumulation in leaves following wounding. PMID: 21143681
- Phenotypic analysis of GASA4 loss-of-function and gain-of-function lines reveals that GASA4 regulates floral meristem identity and positively affects both seed size and total seed yield. PMID: 17284469
- Functional characterization of the GASA4 gene has demonstrated that heat induces its expression. PMID: 17761429
