GASA5 Antibody
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Description
Flowering Regulation
GASA5 acts as a negative regulator of gibberellin (GA)-mediated processes:
| Parameter | GASA5 Null Mutant (gasa5-1) | GASA5 Overexpression | Wild Type Control |
|---|---|---|---|
| Flowering Time | 2–3 days earlier | 4–5 days delayed | Baseline |
| Stem Growth Rate | 15–20% faster | 10–12% slower | Baseline |
Mechanistically, GASA5 modulates:
Stress Adaptation
GASA5 contributes to redox homeostasis during:
Key Experimental Findings
Antifungal Activity Correlation
While direct evidence for GASA5 is limited, homologs like TdGASA1 show:
| Fungal Pathogen | Inhibition Zone (mm) | MIC (mg/mL) |
|---|---|---|
| Fusarium graminearum | 20 ± 0.4 | 0.312 ± 0.6 |
| Alternaria alternata | 18 ± 0.6 | 1.25 ± 0.6 |
Data adapted from durum wheat homolog studies
Technical Considerations
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Antibody Validation: Requires cross-reactivity tests across plant species due to high sequence conservation .
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Assay Compatibility: Works in Western blot (reducing conditions), immunoprecipitation, and fluorescence microscopy .
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Limitations: False positives possible in yeast two-hybrid systems without C-terminal truncations .
Future Directions
Product Specs
Buffer
Preservative: 0.03% ProClin 300
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
14-16 weeks (Made-to-order)
Synonyms
GASA5 antibody; At3g02885 antibody; F13E7Gibberellin-regulated protein 5 antibody; GAST1 protein homolog 5 antibody
Target Names
GASA5
Uniprot No.
Target Background
Function
GASA5 is a gibberellin-regulated protein that negatively regulates gibberellin-induced flowering and stem growth. It may inhibit flowering and inflorescence growth through a pathway involving GAI, by upregulating *FLC* expression, and downregulating *FT* and *LFY* expression. Furthermore, GASA5 acts as a negative regulator in thermotolerance by modulating both salicylic acid (SA) signaling and heat shock protein accumulation.
Gene References Into Functions
- GASA5 suppresses gibberellin responses in *Arabidopsis* (PMID: 24157610)
- GASA5 acts as a negative regulator in thermotolerance in *Arabidopsis*, regulating salicylic acid signaling and heat shock protein accumulation (PMID: 21835493)
- GASA5 functions as a negative regulator of GA-induced flowering and stem growth (PMID: 19190987)
Database Links
Protein Families
GASA family
Subcellular Location
Secreted. Secreted, cell wall. Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Expressed in roots, root hairs, vasculature of cotyledons and hypocotyls, shoot apex, leaf veins, stems, flower receptacles, pollen, filaments, anthers and siliques.
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