GASA6 Antibody

GASA6 in Plants: A Hormone-Responsive Protein

GASA6 is a small, GA-stimulated protein in Arabidopsis thaliana that integrates gibberellin (GA), brassinosteroid (BR), and abscisic acid (ABA) signaling. Key findings include:

• Function: Promotes seed germination by regulating cell wall loosening at the hypocotyl-radicle transition zone via expansins (e.g., EXPANSIN 1) .

• Regulation: Activated by transcription factors HBI1 and BEE2, which bind to the GASA6 promoter’s E-box elements . Overexpression of GASA6 accelerates flowering, while suppression delays it .

• Subcellular Localization: Signal peptide cleavage is critical for proper localization, as confirmed by immunological and imaging analyses .

Antibody Availability: No commercial antibodies for GASA6 are documented in the literature. Research relies on qRT-PCR, GUS reporter assays, or protein expression in E. coli .

Gas6 Antibody (Human): Applications and Research Insights

Gas6, a vitamin K-dependent protein, activates Axl, Tyro3, and Mer tyrosine kinase receptors, influencing cell survival, adhesion, and migration. Below is a detailed analysis of Gas6 antibodies:

Research Findings with Gas6 Antibodies

• Role in Pathways: Gas6/AXL signaling regulates platelet activation, hepatic regeneration, and viral entry (e.g., Dengue, Vaccinia) via apoptotic mimicry .

• Therapeutic Implications: Neutralizing antibodies like CNTO300 block Gas6-receptor interactions by binding the LG1 domain, inhibiting cell survival in cancer models .

• Western Blot Validation:

  • H1299 NSCLC cells: Gas6 expression detected at 64 kDa; treatment with recombinant Gas6 induces Axl phosphorylation and cell migration .

  • SH-SY5Y neuroblastoma cells: Gas6 detected in lysates, confirming tissue-specific expression .

Comparative Analysis of Gas6 Antibodies

GASA6 vs. Gas6: Critical Distinction