Customize GASA7 Antibody

Description

Clarification of Terminology

The term "GASA7" appears in one source ( ), which describes a Gibberellin-regulated protein (UniProt entry O82328) in Arabidopsis thaliana. This protein is unrelated to antibodies or immunological functions. No antibodies targeting this protein or sharing the "GASA7" designation are mentioned in any of the provided materials.

Potential Misinterpretations

Typographical Errors: The term "GASA7" may be a misspelling or misannotation. For example:
- "4A7": A monoclonal antibody targeting Claudin18.2 in gastric and pancreatic cancers (Source ).
- "IgA" or "IgG": Antibody isotypes discussed in Sources , , and .
Domain-Specific Nomenclature: Some antibodies are named based on target antigens (e.g., HER2-targeting Trastuzumab in Source ), but "GASA7" does not align with known antigen nomenclatures.

Research Gaps and Recommendations

The absence of "GASA7 Antibody" in peer-reviewed literature or clinical databases suggests:

Non-existent Compound: The term may refer to a preclinical or proprietary antibody not yet published.
Specialized Field: Potential relevance in niche areas (e.g., plant immunology) not covered in the provided sources.
Synthetic Constructs: No data on engineered antibodies (e.g., bispecific antibodies, ADCs) with this designation.

Actionable Steps:

Verify Terminology: Confirm the spelling and context of "GASA7 Antibody" with the requester.
Expand Search Parameters: Include non-English sources, patents, or preprint repositories (e.g., bioRxiv) for emerging research.
Consult Specialized Databases: Search plant immunology or agricultural biotechnology databases for "GASA7" interactions.

Related Antibody Research (For Context)

While "GASA7 Antibody" is unverified, the provided sources highlight advancements in antibody engineering and applications:

Antibody Type	Key Findings	Source
Claudin18.2-targeting	4A7 monoclonal antibody outperforms IMAB362 in binding affinity and cytotoxicity.
Trastuzumab Biosimilars	RNA-seq confirms functional similarity between originator and biosimilar antibodies.
IgA Antibodies	Preferentially target mucosal surfaces, with limited viral reactivity.	,

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

GASA7 antibody; At2g14900 antibody; T26I20.6Gibberellin-regulated protein 7 antibody; GAST1 protein homolog 7 antibody

Target Names

GASA7

Uniprot No.

O82328

Target Background

Function

GASA7 Antibody targets a gibberellin-regulated protein that may play a role in hormonally controlled developmental processes such as seed germination, flowering, and seed maturation.

Database Links

KEGG: ath:AT2G14900

UniGene: At.40482

Protein Families

GASA family

Subcellular Location

Secreted.

Q&A

What experimental validation methods ensure antibody specificity for Galectin-7 in Western blotting?

Methodological Approach:
- Use dual validation techniques:
  - Western Blot: Employ reducing conditions (e.g., Immunoblot Buffer Group 1) with 1 µg/mL antibody concentration. Expect a band at ~15 kDa in human skin tissue lysates .
  - Simple Western™: Detect at ~21 kDa in HEK001 keratinocytes using 10 µg/mL antibody under reducing conditions .
- Validate against negative controls (e.g., unmodified DNA or unrelated nucleosides) to confirm lack of cross-reactivity .

How do researchers address variability in antibody sensitivity across experimental platforms?

Data Contradiction Analysis:
- Compare results from traditional ELISA (50% inhibition at 54 pmol 7HEG) versus fluorescence-enhanced ELISA (3-10x sensitivity improvement) .
- For Galectin-7, reconcile discrepancies in molecular weight detection (15 kDa vs. 21 kDa) by verifying reducing conditions and tissue/cell line specificity .

What strategies improve the functional assessment of antibody-mediated cellular responses in cancer models?

Integrated Workflow:
- Binding Assays: Validate tumor targeting using ELISA or immunofluorescence (e.g., MG7-scFv/SEB binding to SGC-7901 gastric cancer cells) .
- Functional Assays:
  - Measure cytokine release (IFN-γ, IL-2) from PBMCs co-cultured with antibody-fusion proteins to assess T-cell activation .
  - Quantify apoptosis via Annexin V staining in antibody-treated tumor cells .

How can researchers optimize antibody-dependent cellular cytotoxicity (ADCC) in stratified epithelia studies?

Experimental Design:
- Use immunohistochemistry (IHC) at 1:500 dilution in human colon tissue to maximize signal-to-noise ratios .
- Combine with siRNA knockdown of Galectin-7 in keratinocytes to confirm specificity in UVB-induced apoptosis assays .

Methodological Challenges and Solutions

Challenge	Solution	Source
Cross-reactivity with dG	Combine HPLC with immunoassay to isolate 7HEG adducts in DNA .
Low antibody titers in TGE	Extend bioreactor runs to 12 days (e.g., MERS-7.7G6 yield: 1.74 g/L vs. 0.77 g/L) .
Glycoform variability	Monitor G0F glycoforms via mass spectrometry post-purification .

Key Research Findings

Galectin-7 Antibody:
- Induces apoptosis in keratinocytes via JNK activation and cytochrome-c release .
- Downregulated in squamous carcinoma, making it a biomarker for epidermal homeostasis .
MG7-scFv/SEB Fusion Protein:
- Targets gastric cancer cells (SGC-7901) with 2× higher PBMC-mediated apoptosis vs. controls .
- Increases IFN-γ production by 8-fold in tumor-bearing rat models .

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GASA7 Antibody