At2g39540 Antibody
Description
Introduction to At2g39540 Antibody
The At2g39540 Antibody is a research-grade reagent targeting the protein encoded by the AT2G39540 gene in Arabidopsis thaliana (thale cress). This antibody is commercially available and primarily used to study gene expression, protein localization, and functional roles in plant biology. Below is a detailed analysis of its structure, research findings, and applications.
Gene and Protein Function
The AT2G39540 gene encodes a GA-regulated protein involved in gibberellin (GA) signaling pathways in Arabidopsis (Table 2) . Gibberellins are plant hormones critical for growth and development.
Comparative Analysis of Arabidopsis Antibodies
The At2g39540 Antibody is part of a broader panel of Arabidopsis-specific antibodies (Table 3) . These reagents enable targeted studies of gene function in plants.
| Antibody Target | Uniprot ID | Gene Function |
|---|---|---|
| At1g47765 | Q9FZF3 | Unknown (hypothetical protein) |
| At4g02733 | Q2V3L6 | Unknown (hypothetical protein) |
| At2g39540 | O80641 | GA-regulated protein |
| At5g47300 | Q9LVS9 | Unknown (hypothetical protein) |
Challenges and Future Directions
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Limited Direct Research:
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Potential for Functional Insights:
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Future work could leverage this antibody to elucidate GA signaling mechanisms, particularly in stress responses or developmental transitions.
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Cross-Species Utility:
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While designed for Arabidopsis, homology to orthologs in other plant species (e.g., rice, wheat) may expand its applications.
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Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
KEGG: ath:AT2G39540
STRING: 3702.AT2G39540.1
Q&A
Researchers investigating At2g39540 (ART1 transcription factor in Arabidopsis thaliana) will encounter these methodological and analytical questions in antibody-based studies:
Advanced Technical Challenges
How to resolve contradictory binding data in ART1-DNA interaction studies?
Scenario: Antibody detects probe binding in EMSA but fails in ChIP-seq. Solutions:
Which multiplex strategies work with anti-ART1 antibodies?
Compatible Alexa Fluor® combinations for co-detection:
Methodological Optimization
How to improve signal-to-noise ratio in ART1 immunolocalization?
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Pre-treatment: 1% Triton X-100 + 10 mM sodium citrate (pH 6.0)
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Blocking: 5% BSA + 2% goat serum (prevents non-specific binding)
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Enhancement: Tyramide signal amplification (TSA) with HRP-conjugated secondaries
What computational tools predict ART1 antibody-epitope interactions?
Data Interpretation Framework
How to distinguish ART1-specific phenotypes from off-target effects?
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Compare three independent art1 alleles
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Rescue assay: Express ART1-GFP under native promoter
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Transcriptomic correlation: RNA-seq of WT vs mutant + anti-ART1 ChIP-seq targets
Key metrics:
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≥70% overlap between DEGs and ART1 binding sites
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Phenocopy rate ≥80% across alleles
